1. Figures

2. Fig. 1 Schematic of a lab-built drop-type chemiluminescence (DCL) system

3. Fig.2 Sample treatment procedure for DCL measurement to determine enrofloxacin and its metabolite in chicken meat
NH2
H2N
Amide bond
BSA
HRP
EDC/NHS
BSA

4. Fig.3 EF-TEM image of synthesized and surface modified magnetic nanoparticles (amine-functionalized nanoparticles); core 10.5±1.7 nm, shell 16.3±1.2nm

5. Fig.4 (a) FT-IR spectrum of amine functionalized nanoparticles; silica and NH2 group functionalized MNP

6. Fig.4 (b) FT-IR spectrum of amine functionalized nanoparticles; enrofloxacin attached MNP.

7. Supplementary DATA

8. Y Y Y Y Y Y Y Y Y Y Y Y S-fig. 1. Experimental procedure for ELISA
Primary antibody Y
Secondary
antibody (HRP)
Enrofloxacin
Substrate
(TMB)

9. S-fig. 2. Calibration curve of Enrofloxacin in chicken sample
Luminol
10mM
H2O2
100mM
Boric acid
P-Iodophenol
1.5mM pH
9.5
LOD (pg/mL) R2
RSD (%)
13.92
0.991
<11.4

10. S-fig 3. Calibration curve of Ciprofloxacin in chicken sample
Luminol
10mM
H2O2
100mM
Boric acid
P-Iodophenol
1.5mM pH
9.5
LOD (pg/mL) R2
RSD (%)
7.26
0.994
<8.31

11. S-fig. 4. Calibration curves of Enrofloxacin and Ciprofloxacin in chicken using ELISA
LOD (ng/mL) R2
RSD (%) 1
0.979
<39.24
LOD (ng/mL) R2
RSD (%) 1
0.987
<24.96