1. PR_7_ИФА PR_8_Проточная цитометрия

2. Антитело

3. Варианты ИФА

4. Схема «Сэндвич» метода ИФА 1. To coat the ELISA platewith diluted capture antibody and incubate overnight at 4°C. 2. Wash the plate wells with ddH 2 O, wash with PBS-Triton twice. 3. Block non-specific binding using 1% BSA/PBS and incubate for 30-60 minutes at RT. 4. Wash plate. Add standards and 100ul of diluted samples to appropriate wells. 5. Incubate for 1 hour at RT. Wash. 6. Add 100ul appropriate dilution of the secondary antibody conjugated with Alkaline Phosphatase (AP) or Horseradish Peroxidase (HRP) and incubate for 1 hour. Wash. 7. Add 100ul of substrate to well and incubate at RT for 1 hour. (Add stopping solution) 8. Read plates on an ELISA microplate reader.ELISA platestopping solutionmicroplate reader

6. Проточная цитометрия Flow cytometry http://www.youtube.com/watch?v=TDRhCWaYRsg

7. Fluorescence Activated Cell Sorting (FACS)

9. Холостая проба