1. Lecture 14 IR & 1H NMR Practice Problem (Lec 13)
Column Chromatography of Plant Pigments
Get Lecture Problem 5, due next class
This Week In Lab:
Ch 7 PreLab Due
Quiz 4
Ch 5 Final Report Due
Next Week in Lab:
Ch 9 PreLab Due
Quiz 5
Ch 6 Final Report Due

3. Column Chromatography of Plant Pigments
Chapter 9
Column Chromatography of Plant Pigments
A two-day experiment
Day One - Extraction & Chromatography of Plant Pigments
Day Two - UV/Vis Analysis of Plant Pigments

4. Extraction of Plant Pigments
Obtain spinach leaves
Grind up leaves with acetone, petroleum ether and CaCO3
Transfer to a separatory funnel
Extract (similar to Ch 5!)
Organic layer contains plant pigments - to be separated via column chromatography

5. Column Chromatography of Plant Pigments
Pack column
Load sample (plant pigment solution/organic layer) to column
Run column & collect fractions in tared flasks
**NEVER let the column to dry**
Always have fresh mobile phase in the column
TLC the fractions
See video on column prep in class.

6. Getting the column ready
Column Chromatography
Of Plant Pigments:
Step 1
Getting the column ready

7. What the column will look like - the colored bands
are the plant pigments!
Running the column &
collecting fractions

8. Evaluating the fractions
Finishing up &
Evaluating the fractions
The different fractions
That contain the
Different pigments
Evaluate by TLC &
By UV/Vis

9. Carotenoids Hydrocarbons
Orange/red/yellow pigments in fruits and veggies
Conjugated p systems
Involved in photosynthesis

10. Chlorophylls Porphyrins Blue/green pigments in plant leaves
Also involved in photosynthesis
Produced by plants in response to sunlight

11. Flavonoids Aromatic compounds
Red, violet, blue, or yellow pigments in plants, fruit, flowers
In fruit and flowers, act as an attractant for insects to distribute pollen and seeds
In leaves, act as sunscreens