1. ATB: MAKE A NEW LOGBOOK ENTRY “PROTEIN NETWORK PRACTICE 2”
GET A SHEET FROM THE FRONT COUNTER—1 PER PERSON
PLANNER: DESIGN YOUR OWN NETWORK DUE FRIDAY
CHECKUP/QUIZ ON FRIDAY
LOGBOOK CHECK AT END OF CLASS TODAY
IF YOU MISSED THIS MONDAY’S CHECKUP—MAKE IT UP! IT TURNS INTO A ZERO AFTER FRIDAY

2. Glucose
Lactose
Lactase
Galactose
Key:
Metabolites
Enzyme reactions

3. Labrador (dog) pigmentation
Yellow pigment
tyrosinase
Tyrosine
Dopaquinone
TRP-2
Brown pigment
TRP-1
Boxes = metabolites
Arrows = enzymes that change
the metabolites
Black pigment

4. Noradrenaline Dopamine Thyroxine Phenylalanine Homogentisic acid
Tyrosine
Tyramine
Thyroxine
Hydroxy-
phenylpyruvate
Dopa
Dopamine
Noradrenaline
Adrenaline
Dopaquinone
Melanin
Phenylpyruvate
Maleyl-
acetoacetate
Prephenate 1 2 4 3 5 6

5. Drosophila Eye Color Pigment Network
PTERIDINE PIGMENTS
OMMOCHROME
PIGMENTS
Molecule A
(colorless)
2-amino-4-
hydroxypterdine
(BLUE) 1
Molecule B
(colorless) 3
xanthopterin
(GREEN-BLUE) 2 9 4
tetrahydroiopterin
ommochrome pigment
(BROWN)
isoxanthopterin
(VIOLET-BLUE) 7 6 5
biopterin
(BLUE)
drosopterin
(ORANGE)
sepiapterin
(YELLOW) 8 10
isosepiapterin
(YELLOW)
Color of fly is result of mixture of all pigments in network
In order to see pigments, they must be transported into specific areas of the eye cells. All the ommochrome and pteridine pigments use the same transport proteins (labeled as protein #10 on the network)

6. Scarlet
White
Wild Type
Brown

7. Drosophila Eye Color Pigment Network
PTERIDINE PIGMENTS
OMMOCHROME
PIGMENTS
Molecule A
(colorless)
2-amino-4-
hydroxypterdine
(BLUE) 1
Molecule B
(colorless) 3
xanthopterin
(GREEN-BLUE) 2 9 4
tetrahydroiopterin
ommochrome pigment
(BROWN)
isoxanthopterin
(VIOLET-BLUE) 7 6 5
biopterin
(BLUE)
drosopterin
(ORANGE)
sepiapterin
(YELLOW) 8 10
isosepiapterin
(YELLOW)
Color of fly is result of mixture of all pigments in network
In order to see pigments, they must be transported into specific areas of the eye cells. All the ommochrome and pteridine pigments use the same transport proteins (labeled as protein #10 on the network)

8. Serotonin
Serotonin is a molecule called a neurotransmitter used by cells in the brain to communicate
Part of its function is to regulate emotions
Abnormally low amounts of serotonin in the brain can cause depression
How is serotonin produced, and how can we use our understanding of it to treat depression?

9. Some enzymes involved in serotonin synthesis
Enzyme: AAAD Substrate: 5-HTP Product: serotonin Enzyme: TPH Substrate: tryptophan Product: 5-HTP Enzyme: MAO Substrate: serotonin Product: 5-HIAA
5-HTP
5-HIAA

10. Draw the enzyme pathway used to build serotonin

11. 5-HTP
AAAD
Serotonin
5-HTP
TPH
MAO
5-HIAA
Tryptophan
5-HIAA