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Lab review – part 3 BIOO 201: Botany. Chromatography Purpose of chromatography - separate pigments due to difference in solubility What pigments? – colors?

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Presentation on theme: "Lab review – part 3 BIOO 201: Botany. Chromatography Purpose of chromatography - separate pigments due to difference in solubility What pigments? – colors?"— Presentation transcript:

1 Lab review – part 3 BIOO 201: Botany

2 Chromatography Purpose of chromatography - separate pigments due to difference in solubility What pigments? – colors? Carotenes Xanthophylls Chlorophyll b Chlorophyll a Anthocyanin

3 Chromatography Correlation between leaf color and pigments? Red leaf of dogwood: Yellow leaf of maple: Green leaf of Bradford pear:

4 Chlorophyll a & b content How measured? Which leaves with most chlorophyll content?

5 Absorption Spectrum Why done? How done?

6 Absorption Spectrum

7

8 Seed germination What is the control for the experiment? What is meant by “normal” germination?

9 Seed germination Percent Germination SalinityTrial 1Trial 2Trial 3Trial 4Trial 5Trial 6Trial 7Trial 8Average 0%100 92100 99.0 0.5%88100 96 8010094.5 1.0%889692487692809683.5 1.5%6476 807276486469.5 2.0%1643612 8413.0 2.5%000000040.5 3.0%000000000.0

10 Seed Germination

11 Root length

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