1. Lab SAFETY

2. Laboratory Safety Rap! http://www.teachertube.com/viewVid eo.php?video_id=194405

3. Physical Chemical Biohazards Three Types of Hazards

4. Physical Hazards Electrical equipment Laboratory instruments Glassware Fire Spills

5. Physical Hazard: Electrical - All electrical equipment should be properly grounded according to code - disconnect electrical equipment from power supply when moving, repairing, etc. (ex. Replacing a light bulb in a microscope) - all electrical cords should be in good repair - do not overload outlets and circuits - do not handle electrical equipment with wet hands - do not use extension cords

6. Physical Hazard Laboratory Instruments - use equipment according to manufactures instructions - equipment that operates at high - speeds such as a centrifuge needs to - be equipped with safety latches - centrifuge lids should not be - lifted until the rotor has come to a - complete stop - unplug equipment that has liquids spilled in it Centrifuge- a machine that separates substances of different densities using centrifugal (rotational) force.

7. Physical Hazard Laboratory Instruments - insulated gloves are to be worn when operating an autoclave - use tongs to remove items from an autoclave - the autoclave door must never be opened unless pressure gauge reads zero psi - when sterilizing liquids in an autoclave the containers must be heat resistant and lids loosely capped. - place liquids to be autoclave on a drip pan to catch overflow Autoclave: An airtight steel vessel used to heat substances and objects under very high pressures. Autoclaves are used in laboratory experiments and for sterilization.

8. Physical Hazard Glassware In the laboratory most glassware has been replaced with disposable materials, although glassware can still be in use. - when heating glassware make sure it is heat-resistant such as Kimax or Pyrex - do not use glassware that is chipped or has cracks - broken glass should be swept up with a broom and dustpan and discarded in a cardboard container

9. Physical Hazard - Fire Personnel in the clinical laboratory need to be trained in the use of fire safety equipment and procedures. There really is no reason to use an open flame in the clinical laboratory, instead use hotplates, microwave ovens, autoclaves, incinerators and slide warmers when using instruments for heating. - Keep loose clothing and hair away from heat - All flammable chemicals need to be stored in a flammables cabinet

10. Physical Hazard - Fire Fires are classified into five different types Type A: ordinary combustibles ex. plastics, paper, wood Type B: flammable liquids ex. chemicals Type C: electrical ex. centrifuge, microscope Type D: combustible metals Type E: Cannot be extinguished

11. Physical Hazard - Fire Fires can be classified as a combination of A,B and C classes. Fire extinguishers use a picture/label system to designate which type of fire they are to be used on. example: A-B, A-B-C An easy acronym PASS is used for fire extinguisher operations: P – pull the pin, A – aim at the base of the fire, S – squeeze the trigger, S – sweep at the base of the fire

12. Chemical hazards are gases, vapors, liquids, or dusts that can harm your body. A clinical laboratory technician may come in contact with chemical hazards during the following: - disinfection - preservation of specimens - transportation of specimens - exposure / inhalation

13. Chemicals present a variety of hazards as they can be: - Flammable - Toxic - Caustic - Corrosive - Carcinogenic - Mutagenic Radioactive

17. A clinical lab technician can come into contact with hazardous chemicals when: - conducting test with reagents - adding preservatives (for ex. 24-hour urines) - spills - mixing chemicals - delivering samples

19. A warning label tells you what class a hazardous product is in and how to work safely with it. HAZMAT - an abbreviation for `hazardous material' used on warning labels.

20. A material safety data sheet – gives you more detailed information about how to work safely with a hazardous chemical. Provides information about all the chemicals in the laboratory. Hazards of the chemical Personal Protective Equipment required Body organs that could be adversely affected following exposure First aid and further medical treatment required

21. Chemical Hygiene Plan

22. Biological Hazard Employees who are most at risk from biological hazards are those who work with animals, plants, in health or child care. Biological hazards are living organisms that can cause sickness or disease in humans and animals.

23. Biological Hazards Examples: Bacteria, viruses, or fungus Infected tissue cultures Human or animal blood or body fluids Recombinant DNA

24. Summary: Can you think of any other examples of the three types of hazards? What about around the house? Around the school? Discuss…