1. Web Tools for Parasite Genome Projects

5. Parasite Functional Classification Main Classes :- Metabolism Energy Cell Growth, Cell Division & DNA Synthesis Transcription Protein Synthesis Protein Destination Transport Facilitation Intracellular Transport Cellular Biogenesis Cellular Communication/ Signal Transduction Cell Rescue, Cell Death and Ageing Ionic Homeostasis Hypothetical Proteins Unclassified Proteins

14. Steps in production of classification pages :- 1) Awk script produces formatted files of protein annotations. 2) Perl script searches through keyword files for each protein and outputs web pages. 3) Web pages checked for obvious errors/misclassifications and new ‘Unclassifieds’ assigned to classes where appropriate. (Script re-run if necessary.) 4) Users’ feedback (PLEASE!!) taken account of and keyword files modified accordingly.

15. 7670085 AF153838 AAF66152 AF153838 BLASTN BLASTP GLYCEROL KINASE (Leishmania braziliensis) Example of formatted sequence annotation :-

16. decarboxylase Decarboxylase DECARBOXYLASE lyase Lyase LYASE Example keyword file for lyases/decarboxylases :-

31. Useful Parasite URLs at EBI :- http://www.ebi.ac.uk/parasites/parasite-genome.html http://www.ebi.ac.uk/blast2/parasites.html http://www.ebi.ac.uk/parasites/proteomes.html http://www.ebi.ac.uk/cgi-bin/parasites/class_search.p http://www.ebi.ac.uk/cgi-bin/parasites/motif_search.p http://www.ebi.ac.uk/parasites/leish.html