1. Bio 328 Immunology Immunoglobulins: structure and Function

2. + - Albumin  globulin  globulin  globulin Origin InflammationNormal Myeloma

3. Arne Tiselius and Elvin Kabat 1939.

4. 2014) 1972 Noble Prize in Medicine.

5. Porter et al. Nisonoff et al. Edelman et al.

6. Goat anti-Human Fab binds to both L- and H-chain; goat antiHuman Fc binds only to H-chain.

21. Angiotensin ll

22. Van der Waals surface contact between angiotensin ll and Fab fragment.

23. Flu virus antigen and antibody

24. Fab and Influenza virus antigen

26. Anti-HIV protease Fab + peptide

27. Fab different anti-hen egg lysozyme Fab different small epitopes

28. UnboundAg-bound Induced-fit

29. UnboundAg-bound Anti-hemagglutinin Fab and peptide.

33. Antibody –Mediated Effector Functions 1.Ab-medicated clearance of pathogens a)Neutralization b)Opsonization c)Complement activation d)ADCC 2.Ab isotypes or classes mediate different functions 3.Fc Receptors

35. How do Antibodies kill Cancer Cells? 1.Binding to cell surface receptors and preventing their activation. 2.Interference with vascularization. 3.Induction of apoptosis. 4.Induction of ADCC. 5.Induction of Complement fixation. 6.Delivery of toxins.

37. IgG, IgA, IgDIgM, IgE C H 1/C H 1 Hinge region C H 2/C H 2 C H 3/C H 3 C H 4/C H 4

46. 1921 K. Prausnitz and H. Kustner 1966 K and Y Ishizaka

57. Immunoreceptor Tyrosine Activation Motifs

58. B-cell Linker Protein Bruton’s tyrosine kinase

59. X-linked agammaglobulinemia

62. The End