1. ABSTRACT  It is well known that the natural plants extracts are beneficial for humans thanks to their antioxidant, anti-inflammatory and immunomodulatory effects. The biological activity of these berries is mainly due to their high content of anthocyanins and other polyphenols. The nanoparticles facilitate the penetration of substances in skin, enhancing their antimitotic, anti- inflammatory and antibiotic properties.  We have investigated the pH influence on the morphology of some new hybrid materials based on gold nanoparticles and natural extracts from fruits of Romanian native plants from Adoxaceae family (Viburnum opulus L. and Sambucus nigra L.). We have chosen the optimal method to get these materials in which gold nanoparticles of 10-80 nm were obtained.  We characterized them by UV-Vis and FT-IR spectroscopy, by TEM and DSC. The hybrid materials have been tested on psoriatic lesions, but only after their non toxicity were determined by means of in vitro and in vivo analysis. The medical results emphasized a remarkable improvement of these lesions. Effect of Natural Extracts pH on Morphological Characteristics of Hybrid Materials Based on Gold Nanoparticles Liliana Olenic 1, Adriana Vulcu 1, Ioana Chiorean 2, Maria Crisan 3, Camelia Berghian Grosan 1, Simina Dreve 1, Luminita David 4, Lucian Barbu-Tudoran 5, Irina Kacso 1, Ioan Bratu 1, Camelia Neamtu 1 and Cezara Voica 1 1 National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Street, Cluj-Napoca, Romania 2 Faculty of Mathematics and Informatics, Babeş-Bolyai –University, Kogălniceanu Street, no.1, Cluj-Napoca, Romania 400084 3 Histology Department “Iuliu Haţieganu” University of Medicine and Pharmacy, Emil Isaac Street no.13, Cluj-Napoca, Romania 4 Univ Babes Bolyai, Fac Chem & Chem Engn, RO-400028 Cluj- Napoca, Romania 5 Faculty of Biology and Geology Babes-Bolyai University, Bilascu Street no.44, Cluj-Napoca Romania EXPERIMENTAL CONCLUSIONS: Gold nanoparticles functionalized with anthocyanins extracted from Viburnum opulus L. and Sambucus nigra L. (AuNPs-C and AuNPs-S) were prepared at different pH (4-11) and were characterized by UV-Vis and FTIR spectroscopy and by TEM (at pH 7-9:diameters between 10-80 nm); There were assessed cytotoxicity tests (of the extract alone and of the functionalized nanoparticles) and the antiinflammatory tests (on keratinocytes treated with non-toxic doses of nanoparticles exposed before to increasing doses of UVB irradiation) for hybrid materials obtained at pH 7 respectively 9.5; The histograms of the skin thickness for 8 patients presented an involution of diseases after treatment, fact that indicates the very good antiinflammatory effect of our nanomaterials. Processes in Isotopes and Molecules (PIM 2013), September 25-27, 2013, Cluj-Napoca, ROMANIA Acknowledgement This work was elaborated in the frame project no 147/2012, through the program “Partnerships in priority areas- PN II”, developed with the support of ANCS, CNDI- UEFISCDI  FT-IR spectroscopy  X-ray Diffraction RESULTS and DISSCUTIONS: Fig. 1 Formulas of majoritar compounds anthocyanins- from natural extracts Fig. 2 TEM images of: AuNPs-C, AuNPs-S and UV-Vis spectra of : AuNPs-C; AuNPs-S Fig. 3 X-ray spectra of nanomaterials Fig. 4 FT-IR spectra of nanomaterials at different pH Fig. 5 Histograms of the skin thickness (in μm) : AuNPs-C, AuNPs-S,  Nanomaterials preparation and characterization  Skin before and after treatment Scheme 1 Reaction scheme for obtaining gold nanoparticles