Download presentation
Presentation is loading. Please wait.
Published byEthan Gallagher Modified over 9 years ago
1
Things to Do Today Observe Unknown gm (-) results Start Urinalysis Start gm (+) ID –Check for hemolysis –Catalase test –Coagulase test –Direct stain colonies Observe Throat cultures –Check for hemolysis –Catalase test –Gm stain “isolated colonies”
2
Oxidase Test Negative Positive
3
Urease Test Negative Positive
4
Methyl Red Test Negative Positive
5
KIA Slants uninoculated
6
KIA Slants uninoculated
7
Citrate test Negative positive
8
The Enterotube Multitest System
9
The API System: Various species of Enteobacteriaceae are shown here with differences in reactions that enable them to be identified
10
Things to Do Today Observe Unknown gm (-) results –Perform oxidase test with culture from KIA slant –Observe Urease tubes –Perform MR test by adding 6-8 drops MR reagent to the culture –Observe KIA slants for the various reactions
11
Three species of Candida can be differentiated in mixed culture when grown on CHROMagar Candida plates
12
Identification of urinary tract pathogens with differential media (CHROMagar)
14
EMB with E. coli Used with permission
15
EMB Pseudomonas auginosa Enterobacter aerogenes
16
Fig. 15-22 Relative Frequencies of Nosocomial Infections I John Wiley
17
Fig. 15-19 Relative Frequencies of Nosocomial Infections II John Wiley
18
Things to Do Today Start Urinalysis –Do a 4 quadrant streak with the urine sample using a metal loop onto the EMB plate –Perform the “$” streak with the urine sample using the plastic loop onto the BAP plate
19
A tale of two Staphs
20
BAP Hemolytic Reactions Used with permission
21
Throat Swab Used with permission
22
CatalaseCatalase test Positive negative
23
Coagulase test Place a drop of the red latix suspension on the card. Mix a tiny portion of the colony into the mix.
24
Things to Do Today Start gm (+) ID 1.Direct stain with Crystal Violet 2.Check for hemolysis 3.Catalase test 4.Coagulase test Observe Throat cultures (Do in this order) 1.Check for hemolysis 2.Catalase test and find cat (-) colony 3.Gm stain “isolated colonies” on slide with throat swab
26
BAP Hemolytic Reactions Alpha hemolysis Beta hemolysis gamma
27
Types of hemolysis: A: Alpha or partial hemolysis of red blood cells results in a greenish zone B: B-hemolysins, which produce complete breakdown of hemoglobin, causing clear zones to form around colonies on blood agar A B
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.