1. Things to Do Today Observe Unknown gm (-) results Start Urinalysis Start gm (+) ID –Check for hemolysis –Catalase test –Coagulase test –Direct stain colonies Observe Throat cultures –Check for hemolysis –Catalase test –Gm stain “isolated colonies”

2. Oxidase Test Negative Positive

3. Urease Test Negative Positive

4. Methyl Red Test Negative Positive

5. KIA Slants uninoculated

6. KIA Slants uninoculated

7. Citrate test Negative positive

8. The Enterotube Multitest System

9. The API System: Various species of Enteobacteriaceae are shown here with differences in reactions that enable them to be identified

10. Things to Do Today Observe Unknown gm (-) results –Perform oxidase test with culture from KIA slant –Observe Urease tubes –Perform MR test by adding 6-8 drops MR reagent to the culture –Observe KIA slants for the various reactions

11. Three species of Candida can be differentiated in mixed culture when grown on CHROMagar Candida plates

12. Identification of urinary tract pathogens with differential media (CHROMagar)

14. EMB with E. coli Used with permission

15. EMB Pseudomonas auginosa Enterobacter aerogenes

16. Fig. 15-22 Relative Frequencies of Nosocomial Infections I John Wiley

17. Fig. 15-19 Relative Frequencies of Nosocomial Infections II John Wiley

18. Things to Do Today Start Urinalysis –Do a 4 quadrant streak with the urine sample using a metal loop onto the EMB plate –Perform the “$” streak with the urine sample using the plastic loop onto the BAP plate

19. A tale of two Staphs

20. BAP Hemolytic Reactions Used with permission

21. Throat Swab Used with permission

22. CatalaseCatalase test Positive negative

23. Coagulase test Place a drop of the red latix suspension on the card. Mix a tiny portion of the colony into the mix.

24. Things to Do Today Start gm (+) ID 1.Direct stain with Crystal Violet 2.Check for hemolysis 3.Catalase test 4.Coagulase test Observe Throat cultures (Do in this order) 1.Check for hemolysis 2.Catalase test and find cat (-) colony 3.Gm stain “isolated colonies” on slide with throat swab

26. BAP Hemolytic Reactions Alpha hemolysis Beta hemolysis gamma

27. Types of hemolysis: A: Alpha or partial hemolysis of red blood cells results in a greenish zone B: B-hemolysins, which produce complete breakdown of hemoglobin, causing clear zones to form around colonies on blood agar A B