1. VACCINOLOGY (BIO-301) Credit Hrs 3(2-1) Dr. Aneela javed

2. VACCINE PRODUCTION

6. CO 2 Incubators Water Jacketed CO 2 incubator 3 Gas/CO 2 Incubator with RH Control – Precise control of Oxygen levels combined with CO 2, N 2 and RH ensure accurate conditions for applications such as, hypoxic cell studies and cancer research.

7. CELL CULTURE

8. CRYO VIALS

9. Revival of frozen cells Growth of the cells in adequate media with 1.serum (FCS/FBS) 2.antibiotics Complete media, Importance of aliquots

10. serum

11. ADHERENT CELL LINES SUSPENSION CELL LINES

12. Environment: – Temperature: 37°C (34 °C, 41 °C) – High humidity – 5% CO 2 Split: Trypsin-EDTA Count of Cells (Thrypan Blue)

13. Laminar Flow HOOD HEPA filter rated at 99.99% efficient for 0.3 micron particulates. The HEPA filtered air is then directed vertically across the work surface.