Presentation is loading. Please wait.

Presentation is loading. Please wait.

Cytoplasmic Regulation of the Movement of E-Cadherin on the Free Cell Surface as Studied by Optical Tweezers and Single Particle Tracking: Corralling and.

Published byStephen Hudson Modified over 9 years ago

Similar presentations

Presentation on theme: "Cytoplasmic Regulation of the Movement of E-Cadherin on the Free Cell Surface as Studied by Optical Tweezers and Single Particle Tracking: Corralling and."— Presentation transcript:

1 Cytoplasmic Regulation of the Movement of E-Cadherin on the Free Cell Surface as Studied by Optical Tweezers and Single Particle Tracking: Corralling and Tethering by the Membrane Skeleton Yasushi Sako, Akira Nagafuchi, Shoichiro Tsukita, Masatoshi Takeichi, and Akihiro Kusumi J. Cell Biol. Volume 140(5):1227-1240 March 2, 1998

2 A tether model (A) and a fence model (B) proposed as mechanisms for the regulation of lateral movements of E-cadherin (Sako and Kusumi, 1995) Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

3 Structures of the wild-type E-cadherin and its artificial mutants used in this study Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

4 Indirect immunofluorescence staining of E-cadherin on the surface of cells transfected with E- cadherin cDNAs Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

5 Trajectories of particle–cadherin complexes dragged by OT Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

6 (A) The time course of a representative dragging experiment Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

7 Distributions of the escape distances Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

8 Distributions of the freely dragged distances Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

9 Distributions of the effective spring constant (kmsk) of the portions of the membrane skeleton/cytoskeleton that are involved in corralling or tethering E-cadherin molecules Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

10 kmsk (effective spring constant) plotted against the freely dragged distance (dfd) for individual particles Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

11 SPT trajectories of E-cadherins recorded for 16.7 s (500 video frames) Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

12 Distributions of Dmicro Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

13 Classification of trajectories into simple Brownian, confined, and directed movement modes in a time window of 5 s Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

14 Typical trajectories of Wild (A) and Fusion (B) classified as directed movement Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

15 Models of the mechanisms for the regulation of the movement of E-cadherins in the plasma membrane Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

Download ppt "Cytoplasmic Regulation of the Movement of E-Cadherin on the Free Cell Surface as Studied by Optical Tweezers and Single Particle Tracking: Corralling and."

Similar presentations

Diffusion and Osmosis.

Diffusion and Osmosis.
Structure and Function

Structure and Function
Optical Tweezers F scatt F grad 1. Velocity autocorrelation function from the Langevin model kinetic property property of equilibrium fluctuations For.

Optical Tweezers F scatt F grad 1. Velocity autocorrelation function from the Langevin model kinetic property property of equilibrium fluctuations For.
PED GENE PRODUCT IS MHC CLASS 1B MOLECULE Qa-2 Qa-2 CONTROLS RATE OF EARLY CLEAVAGE DIVISION Qa-2 INFLUENCES SUBSEQUENT EMBRYO SURVIVAL HUMAN EMBRYOS HAVE.

PED GENE PRODUCT IS MHC CLASS 1B MOLECULE Qa-2 Qa-2 CONTROLS RATE OF EARLY CLEAVAGE DIVISION Qa-2 INFLUENCES SUBSEQUENT EMBRYO SURVIVAL HUMAN EMBRYOS HAVE.
Tre1 GPCR initiates germ cell transepithelial migration by regulating E-cadherin Prabhat S. Kunwar, Hiroko Sano, Andrew D. Renault, Vitor Barbosa, Naoyuki.

Tre1 GPCR initiates germ cell transepithelial migration by regulating E-cadherin Prabhat S. Kunwar, Hiroko Sano, Andrew D. Renault, Vitor Barbosa, Naoyuki.
HISTOLOGY “What is that icky little tissue?”. Not All Cells Are Alike: Multi-Cellular Organisms: Require Cellular Specialization JOB BREAKDOWN: Coverings.

HISTOLOGY “What is that icky little tissue?”. Not All Cells Are Alike: Multi-Cellular Organisms: Require Cellular Specialization JOB BREAKDOWN: Coverings.
Membranes and Transport Chapter 6. 6.1 Membrane Structure  Biological membranes contain both lipid and protein molecules  Fluid mosaic model explains.

Membranes and Transport Chapter Membrane Structure  Biological membranes contain both lipid and protein molecules  Fluid mosaic model explains.
 1972 Singer and Nicolson developed the fluid mosaic model Main Function: Isolate the cell’s contents from the external environment and regulate what.

 1972 Singer and Nicolson developed the fluid mosaic model Main Function: Isolate the cell’s contents from the external environment and regulate what.
Let’s look at one example involving osmosis. Osmosis is the diffusion of water across a semi permeable membrane such as a cell membrane. A semi permeable.

Let’s look at one example involving osmosis. Osmosis is the diffusion of water across a semi permeable membrane such as a cell membrane. A semi permeable.
1 States of Matter Chemistry States of Matter. 2 The Four States of Matter Four States  Solid  Liquid  Gas  Plasma.

1 States of Matter Chemistry States of Matter. 2 The Four States of Matter Four States  Solid  Liquid  Gas  Plasma.
 A physical property of matter.  Solid  Liquid  Gas.

 A physical property of matter.  Solid  Liquid  Gas.
The Plasma Membrane Examples of different membrane proteins include  Ion channels  Carriers  Receptors.

The Plasma Membrane Examples of different membrane proteins include  Ion channels  Carriers  Receptors.
Magnetic Tweezer System Development Jason Sherfey Senior BME, Vanderbilt University Probing mechanical properties across multiple scales Advisor: Dr. Franz.

Magnetic Tweezer System Development Jason Sherfey Senior BME, Vanderbilt University Probing mechanical properties across multiple scales Advisor: Dr. Franz.
Structural Organization Atoms Simple molecules Macromolecule s Membranes Organelles Cells Tissues Organs Organ Systems Animal Small Large.

Structural Organization Atoms Simple molecules Macromolecule s Membranes Organelles Cells Tissues Organs Organ Systems Animal Small Large.
Diffusion and Osmosis. Passive Transport Passive transport- movement of molecules across a cell membrane without energy input Refresh: Solute Object being.

Diffusion and Osmosis. Passive Transport Passive transport- movement of molecules across a cell membrane without energy input Refresh: Solute Object being.
Passive Transport transport of molecules across the cell membrane that does not require energy! 3 Types: 3 Types: 1) Diffusion 2) Osmosis 3) Facilitated.

Passive Transport transport of molecules across the cell membrane that does not require energy! 3 Types: 3 Types: 1) Diffusion 2) Osmosis 3) Facilitated.
Transport The movement of molecules and ions across a membrane.

Transport The movement of molecules and ions across a membrane.
3.6 How Do Diffusion And Osmosis Affect Transport Across The Plasma Membrane? Simple diffusion through the phospholipid bilayer Fig. 3-7a Simple diffusion.

3.6 How Do Diffusion And Osmosis Affect Transport Across The Plasma Membrane? Simple diffusion through the phospholipid bilayer Fig. 3-7a Simple diffusion.
CHAPTER 7.4 & 7.5 ACTIVE TRANSPORT ENDOCYTOSIS & EXOCYTOSIS.

CHAPTER 7.4 & 7.5 ACTIVE TRANSPORT ENDOCYTOSIS & EXOCYTOSIS.
Passive and Active Transport Biology I. Main Idea Cellular transport moves substances within the cell and moves substances into and out of the cell.

Passive and Active Transport Biology I. Main Idea Cellular transport moves substances within the cell and moves substances into and out of the cell.

Similar presentations

Ads by Google