1. Muhammad Ashfaq Pakistan barcode project: progress and applications

2. Pakistan Agro-based economy but crops are plagued with pests and diseases

3. 2010: Barcoding economically important insect species from Pakistan Biodiversity Institute of OntatioNational Institute for Biotechnology and Genetic Engineering (NIBGE) Funded by: HEC

4. Project execution Involvement of universities/ research institutesMuseumsFaculty/ scientistsGraduate students

5. Manual catching Pipettes and sieves Arial nets Light traps Shaking onto sheets Activities and Progress Collecting animal parasites

6. Specimen preparation and databasing

10. Specimens Animals: 34103 Plants: 1289 Barcodes Animals: 28605 Plants: 988 IDRC (62%) 18 months

11. Pests Beneficials

12. Pakistan-only BINs/ species I.Animals: 4021 II.Plants: 363 Number of BINs/ species 2793 (70%)Singletons: 1530

13. I.Families: 199 II.Genera: 698 Taxonomic identifications Unidentified: 22308 III.Species: 764

14. Barcode Applications Insect pests II.Identification and distribution of:I.Barcode reference libraries Disease vectors

15. Reference libraries Pests:754 speciesBeneficials:241 species

16. Noctuid species in/around cotton fields of Punjab Previously only S. litura was reported from cotton in Punjab

17. Genetic diversity: mango mealybug, Drosicha mangiferae

18. Bemisia tabaci, a complex of cryptic species

19. (A) Asia II 1 (B) MEAM 1 (C) Asia 1 Bemisia tabaci, distribution analysis

20. Dengue epidemic in Punjab

21. Mosquito species in Punjab

22. Genus: Anopheles Genus: Culex Genus: Aedes Aedes aegypti Aedes albopictus Genus-wise distribution of mosquitoes in Punjab

23. Outreach Training and involvement of communitySymposiums and seminarsOne-to-one meetingsMedia coverage

26. Barcoding project at NIBGE Strengths A trained group of workersSpecimen processing facilityDepository for voucher specimens

28. Concerns No imminent financial support to continue barcoding activities after August 2013

29. Barcoding team members

30. Thank you for your attention