1. Advances in Hydrocarbon Remediation Technology and Water Treatment
Ben Lee, Refine Innovations, LLC

2. Advances in Hydrocarbon Remediation Technology and Water Treatment
Benjamin Lee -- Refine Innovations, LLC

3. Contaminant Removal: “Dig and dump” Mixture: “Solution by Dilution”
© 2015 Refine Innovations, LLC Proprietary and Confidential
Common Remediation Methods
Contaminant Removal: “Dig and dump”
Mixture: “Solution by Dilution”
Flash off: “Land-farming”
Thermal/Incineration
Bioremediation -- Bacillus

4. Classical Bioremediation: Bacillus
© 2015 Refine Innovations, LLC Proprietary and Confidential
Classical Bioremediation: Bacillus
Spore
Bacillus Microbe
Two general bioremediation categories:
Bio-stimulation
Bio-augmentation

5. Typical Bacillus Performance
© 2015 Refine Innovations, LLC Proprietary and Confidential
Classical Bioremediation: Bacillus
Typical Bacillus Performance

6. Advanced Bioremediation: Pseudomonas
© 2015 Refine Innovations, LLC Proprietary and Confidential
Advanced Bioremediation: Pseudomonas
Pseudomonas Culture

7. Typical Pseudomonas Performance
© 2015 Refine Innovations, LLC Proprietary and Confidential
Advanced Bioremediation: Pseudomonas
Typical Pseudomonas Performance

8. Pseudomonas Treatment
© 2015 Refine Innovations, LLC Proprietary and Confidential
Pseudomonas Treatment
Pseudomonas Treatment is a Bio-augmentation process and involves 3 components:
Biological (Culture, transport, and apply Pseudomonas Microbes at high concentrations)
Chemical (Bio-surfactant)
Mechanical (Application equipment that ensure even and complete contact)

9. Bio–Surfactant Chemistry
© 2015 Refine Innovations, LLC Proprietary and Confidential
Chemistry
Bio–Surfactant
Allows microbes to move more easily through the contaminated site
Specialized chemistry does not harm pseudomonas
Highly biodegradable (90% degraded in 8 days)

10. Earth Cleaning Machine Microbial Mixing Trailer
© 2015 Refine Innovations, LLC Proprietary and Confidential
Mechanical
Earth Cleaning Machine
Treated Soil Conveyor
Application equipment ensures successful remediation in a one-time application:
Calibrated Earth Cleaning Machine to ensure the pseudomonas microbes are applied evenly and effectively to all contaminated soil.
Prepped Mixture Tanks
Microbial Mixing Trailer

11. Earth Cleaning Machine
© 2015 Refine Innovations, LLC Proprietary and Confidential
Earth Cleaning Machine

12. Treated Material Treated Material
© 2015 Refine Innovations, LLC Proprietary and Confidential
Treated Material
Treated Material

13. Case Study: Soil Application 1
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Soil Application 1
This graph shows results for a soil application performed recently in the southwest U.S.  This data was obtained by an independent party, taking samples on a site treated during remediation operations on a contaminated site in North America.

14. Case Study: Soil Application 2
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Soil Application 2
This graph shows results for a soil application, broken out by hydrocarbon chain length.  This data was obtained by an independent party, taking samples on a site treated during remediation operations on a contaminated site in North America.

15. Case Study: Soil Application 3
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Soil Application 3
This graph shows results for a sludge application conducted in Texas during Summer, Data is broken out by hydrocarbon chain length.  This data was obtained by an independent party, taking samples on a site treated during remediation operations on oilfield sludge, tank bottoms, and drilling cuttings.

16. Case Study: Frac Pond, South Texas
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Frac Pond, South Texas
Background: Client had a Hydrocarbon (TPH and BTEX) Contaminated Frac Pond in South Texas. Pond was treated with pseudomonas: TPH and BTEX tested out at non-detect 10 days later. This project saved the client 80% over their next closest remediation option.
Before
After

17. Case Study: Freshwater Application
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Freshwater Application
This table shows the before and after contamination levels for a contaminated freshwater lake. This data was also independently obtained on a site treated in North America. Our product also works in salt water environments.

18. Case Study: Freshwater Application
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Freshwater Application

19. Case Study: Freshwater Application
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Freshwater Application
Pre Treatment Post Treatment

20. Case Study: Freshwater Application
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Freshwater Application
Pre Treatment Post treatment
Water and soil are now treated, allowing natural state to return.

21. Case Study: Freshwater Application
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Freshwater Application
Pre Treatment Post Treatment

22. Case Study: Freshwater Application
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Freshwater Application

23. Case Study: Freshwater Application
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Freshwater Application

24. Successful at Degrading:
© 2015 Refine Innovations, LLC Proprietary and Confidential
Successful at Degrading:
Dichlorobenzene
Dichlorotoluene
Methyl Ethyl Ketone
Methylene Chloride
Naphthalene
Fluorine
Benzene
Toluene
Ethylbenzene
Xylene
Crude oils / LCM
Petroleum
Isoprenoids
Limonene
Citronellal
Chlorinated Solvents
Aliphatic Hydrocarbons
Creosote
Amines (DEA/etc…)

25. Overview of Technology
© 2015 Refine Innovations, LLC Proprietary and Confidential
Overview of Technology
FEATURES:
All components are TSCA, and EPA NCP approved
Proven biological system that degrades organics to carbon dioxide and water
Readily biodegradable, water based formula
Pathogen free
DNA tested
May be shipped non-regulated world wide by any means
BENEFITS:
Total hydrocarbon degradation
Eliminate costly transportation and landfill charges, especially in remote sites
Reduce liability: removal of dozens of trucks off the roads vs “dig and dump” remediation.
Eliminate future potential liabilities from landfill containment failure
Significant dollars savings per project
Success achieved within days/months rather than years

26. Applications Oil Spills Land Water Ground Water
© 2015 Refine Innovations, LLC Proprietary and Confidential
Applications
Oil Spills
Land
Water
Ground Water
Brownfields (Retail and Bulk Fuel Stations)
Underground storage tanks
Abandoned Property
Oil and Gas Industry
Drill Cuttings
Soil
Sumps
Tank Bottoms
LCM
Flow-back and Produced Water Treatment

27. Benjamin Lee: Ben@refine-innovations.com
© 2015 Refine Innovations, LLC Proprietary and Confidential
Contact
Benjamin Lee:
Phone:
16301 Interstate 27, Suite B
Canyon, TX 79015

28. Return completed forms at the conclusion of the conference.
Please remember to fill out the conference survey. This will be sent to you by .
Conference participants are eligible for up to 13 contact hours 1.3 CEUs.  Forms will be available after lunch on Wednesday at the registration are.
Return completed forms at the conclusion of the conference.

29. Advances in Hydrocarbon Remediation Technology and Water Treatment
Ben Lee, Refine Innovations, LLC