1. Measles and Immunity Lab A peek into the Vaccination & Immunity Module created and produced by the Health and Science Pipeline Initiative (HASPI) in collaboration with Rady Children’s Hospital, the Jonas Salk Legacy Foundation, the San Diego Rotary, and the San Diego Foundation.

2. What is in the Module? Background Information Video – A Shot to Save the World Video – Invisible Threat Measles and Immunity Lab Probability Activity Safety & Effectiveness Research Campus Survey Discussion Activity Vaccination Education Project

3. Benefits NGSS & CCSS aligned resources Both videos Lab supplies Guest speaker Curriculum support Benefits NGSS & CCSS aligned resources Both videos Lab supplies Guest speaker Curriculum support Requirements Complete application Module completion Spring 2016 Instructor evaluation Student evaluations Requirements Complete application Module completion Spring 2016 Instructor evaluation Student evaluations

4. Measles & Immunity Lab Split your table into two lab groups Each group will need: one 96-well plate two plastic pipettes Share: IgM Test IgG Test 0.1 M Hydrochloric Acid 0.1 M Sodium Hydroxide

5. Each 96-well plate will test 48 subjects Rows A – D are for the IgM Test (infection antibodies) Rows E – H are for the IgG Test (memory antibodies) Wells A1 and E1 are for the same subject

6. Prepping the Plates – pgs. 3 - 4 Rows E-H Add a drop of Sodium Hydroxide to 35 – 45 wells Positive wells indicate IMMUNITY The IgG Test is phenolphthalein Rows E-H Add a drop of Sodium Hydroxide to 35 – 45 wells Positive wells indicate IMMUNITY The IgG Test is phenolphthalein Rows A-D Add a drop of Hydrochloric Acid to 5 – 10 wells Positive wells indicate INFECTION IgM Test is bromothymol blue Rows A-D Add a drop of Hydrochloric Acid to 5 – 10 wells Positive wells indicate INFECTION IgM Test is bromothymol blue

7. Perform the Tests – pg. 8 The IgM Test Using a plastic pipette add a drop of IgM Test to every well in Rows A-D The IgM Test Using a plastic pipette add a drop of IgM Test to every well in Rows A-D The IgG Test Using a plastic pipette add a drop of IgG Test to every well in Rows E-H The IgG Test Using a plastic pipette add a drop of IgG Test to every well in Rows E-H

8. Results – pg. 9

10. Thank you for attending! Leave your plates and supplies on the table Please do not forget to: Turn in your evaluation Pick up and complete the substitute reimbursement form if needed Pick up a Measles & Immunity Lab kit Complete a Vaccination & Immunity Module Application if interested