1. Epigenetics: Investigating the Structure & Function of Deoxyribonucleic Acid

2. Interdisciplinary Investigations (IDI)
Chemistry 161
Chemistry 162
Chemistry 163
Epigenetics
This Investigation:
Day 1
Pre-Lab Discussion
Part 1: Structure and Function of DNA
Part 2: IDI Challenge 1
Day 2
Part 3: Seminar
Part 4: IDI Challenge 2
Part 5: Post-lab Assignment

3. What did you find most interesting about each video?
Pre-Lab Discussion
What did you find most interesting about each video?

4. Part 1 A-C: Parts of the DNA Molecule
Sugar
Nitrogenous Bases

6. Polarity of G & C Bases

7. D. Linking Through Chemical Bonds

8. The Phosphodiester Bond

9. DNA Double Helix

10. E. Functions of DNA DNA Replication: Gene Expression:
DNA must be accurately copied to allow for the production of new cells during growth, repair, and reproduction.
Gene Expression:
Specific regions of DNA, called genes, must convey information to allow cells to build the needed proteins through transcription and translation.

11. Functions of DNA
DNA Replication:

12. DNA Replication: Let’s Watch

13. Functions of DNA
Gene Expression

14. Epigenetics and DNA Methylation:
Do all your cells have the same DNA?
Do all your cells use all their DNA? Why or why not?

15. All Our Cells Work From Essentially the Same Genome/DNA Template.
Thus cells must be able to regulate which genes they use.

16. Epigenetics and DNA Methylation:
Differential gene expression allows different cell types to adopt different fates.
Mistakes in gene regulation are associated with a variety of diseases, especially cancer.

17. Gene Regulation Plays an Important Role in Many Diseases

18. DNA Methylation DNA methylation silences genes.
This reaction is enzyme-mediated.
DNA methylation silences genes.
Write down your ideas for the three questions in Part E!

19. Part 2: IDI Challenge
On your white board, make a group drawing that represents your thinking about how methylation of DNA “silences” a gene?
Be sure to capture your drawing as a photograph for your post-lab report!

20. Part 3: Seminar Assignment For Next Week: Read these two articles:
“A New Kind of Inheritance”, Scientific American, 2014
“Ancestral dichlorodiphenyltrichloroethane (DDT) exposure promotes epigenetic transgenerational inheritance of obesity” BMC Medicine, 2013.
2. Write a summary of the articles.
3. Write three questions
4. Bring the summary and questions with you to class and be prepared to participate in an in-class seminar

21. Day 2 Agenda: Intro to Epigenetics The Structure & Discovery of DNA
DNA Function
Methylation
Drawing Activity

22. How do we detect DNA methylation?
We use CHEMISTRY!

23. How do we detect DNA methylation?
We use CHEMISTRY!

24. Next: Your Seminar Find your team! Introductions and Roles.
Discuss the paper, using your questions as a guide.
Report out!

26. Structure, Function, and the Value of Visualization:

27. Challenge: Let’s Draw Again!
On your white board, make a drawing that represents your thinking about how methylation of DNA “silences” a gene?

28. DNA Methylation: Let’s Watch