1. Chapter 10 Gene Isolation and Manipulation
Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse.

2. Finding specific nucleic acids by using gel electrophoresis and blotting

9. Polymerase chain reaction (PCR)

14. Sequencing

15. The structure of 2’,3’-dideoxynucleotides
Sanger Sequencing (Dideoxy Sequencing)
The structure of 2’,3’-dideoxynucleotides

18. The dideoxy sequencing method

19. Reading the DNA sequence from an automatic sequencer

20. How to amplify an interesting gene

21. Making a cDNA clone

28. Introduction of a transgene

33. Agrobacterium tumefaciens
causal agent of Crown Gall

34. The Ti plasmid vector

35. The generation of a transgenic plant

36. The generation of a transgenic plant

37. Pattern of transmission of T-DNA