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The MYSTERY of the altered GENE Professor

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Presentation on theme: "The MYSTERY of the altered GENE Professor"— Presentation transcript:

1 The MYSTERY of the altered GENE www.cilr.uq.edu.au Professor
Peter M Gresshoff

2 www.cilr.uq.edu.au NODULE FORMATION NITROGEN FIXATION and Professor
Peter M Gresshoff

3 LEGUMES form NODULES Nodules 1 mm Sucrose Nitrogen Gas Ammonium
Amino acids/protein/DNA/RNA

4 ENERGY: N2 + 16 ATP + 8 e- + 8 H+ 2 NH3 + 16 ADP + H2
Solar (photosynthesis) or fossil (Haber-Bosch process)????

5 deformation and curling
Nodulation and nitrogen fixation are key processes for food, feed, and fuel Nodule induction Colonisation Endosymbiosis Root hair deformation and curling Nitrogen Fixation Protein-rich Seeds Cell Division

6 Nod factors Nod genes RECEPTION NFR1/5 Rhizobium FLAVONES Legume Root

7 Root hairs Rhizobia

8 Curling root hair )

9 Infection pocket Cell walls Infection thread

10 Cell nuclei Inner cortex cells dividing Vascular bundle

11 Infection thread Rhizobia in cortex

12 Nodule primordium Epidermis Xylem Cortex Phloem

13 Bacteroids Vasculature

14 Nodule cortex Nucleus Rhizobium cells (25,000)

15 N2 + 16 ATP + 8 e- + 8 H+ 2 NH3 + 16 ADP + H2 The NODULATION process
allows the conversion of atmospheric nitrogen gas to ammonia - which is a fertiliser for all plants malate amino acids Bacteroid within a legume nodule cells, engulfed by a plant membrane controlling nutrient and ammonia transport N ATP + 8 e- + 8 H+ 2 NH ADP + H2 ammonia

16

17 ? ? ? ? ?

18 Thanks to Uli, Brett, Akira, Igor and others for photos

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