1. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP meeting Leafy Vegetables WP 3 Pascal COQUIN GEVES : Variety and seed, study and control group Groupe d’Etude et de contrôle des Variétés Et des Semences

2. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 25/12/2015 A.Objectives  Evaluation for pests and diseases resistance  Evaluation for quality and abiotic characters  Inclusion in the databases to enable a more sustainable production methods and a better quality product

3. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 Deliverables for WP3 Deliverable date WP3 Deliverable description StatusPartner responsible Year 1 D16; month 12 Seeds of the selected material of the four crop groups sent to the partners involved Done except for P12 P0, P1, P2, P3, P4, P5, P7, P9, P10, P12, P13 Year 4 D17; month 45115 accessions of lettuce evaluated Not yet dueP1, P4, P7, P9, P10, P13 D18; month 45320 accessions of spinach evaluated Not yet dueP0, P5 D19; month 45265 accessions of chicory evaluated Not yet dueP2, P12 D20; month 4549 accessions of minor crops evaluated Not yet dueP3, P5, P12 D21; month 45 Evaluation data entered in a fixed format to be sent to the database managers Not yet dueP0, P1, P2, P3, P4, P5, P7, P9, P10, P12, P13

4. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 Milestones for WP3 Milestone date Milestone descriptionStatusPartner responsible Year 1 month 12 Material selected for the evaluation activities of the four crop groups (see WP1) Done except for P12 P0, P1, P2, P3, P4, P5, P7, P9, P10, P12, P13 Year 2 month 18Evaluations started On goingP0, P1, P2, P4, P5, P7, P9, P10, P12, P13 Year 3 month 3670 accessions of lettuce evaluated Not yet dueP1, P4, P7, P9, P10, P13 month 36250 accessions of spinach evaluated Not yet dueP0, P5 month 36170 accessions of chicory evaluated Not yet dueP2, P12 month 36 30 accessions of minor crops evaluated Not yet dueP3, P5, P12 Year 4See deliverables Not yet due

5. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 Milestones in 2010 of evaluation for pests and diseases resistance  Lettuce: 60 accessions –Bremia lactucae :  P4 (CZ): 30 accessions with 20 isolates by seedling tests,  P9 (GBR): 17 accessions for field resistance –Root-knot nematodes (Meloidogyne sp.) : P10 (SLO): 10-20  Spinach: 250 accessions (3-5 races) –Downy mildew (Peronospora farinosa) P0 (NLD) : 250  Chicory: 165 accessions –Alternaria cichoriiP2 (FRA): 60 –Sclerotinia sclerotiorum P2 (FRA): 30 –Thielaviopsis basicolaP2 (FRA): 75

6. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 Milestones in 2010 of evaluation for quality and abiotic characters  Lettuce: 45? (or less) instead of 55 accessions? –Post harvest discolorationP1(GBR): 24 –Winter hardinessP7 (AUT): 6 instead of 15 –TasteP7 (AUT): 6  Spinach: 70 accessions –Nitrate, oxalic acid, dry matterP5 (DAN, SW)  Chicory: 100 accessions –SesquiterpenesP9 (ITA): 100  Rucola: 49 accessions –Nitrate, dry matter P5 (DAN, SW): 20 –Glucosinolate contentP9 (ITA): 20 to 40

7. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP3 : what has been done in 2008 Pests & diseases resistance + Quality & abiotic characters evaluation 7/14 16/17 28/24 30 acc. 3/20 str. 6/6 51/70 263/250 20% (53/265) 37 20 136% (57/49) 83% ( 87/105 ) 99% ( 318/320 )

8. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 WP3 : what will be done in 2009 Pests & diseases resistance + Quality & abiotic characters evaluation 105 inst. of 115 P4: 30 acc. ? str. of B.l.? 57 57 >

9. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 25/12/2015 Evaluation for P0 (CGN)  What has been done : - Evaluation of 263 spinach accessions for 2 P. farinosa pathotypes resistance (Pfs 5 and Pfs 7) - Send the results of Pfs5 and Pfs7 to WP3 leader at the end of 2008 - Include the data in the common database,  What will be done in 2009 : - Evaluation of the 263 spinach accesions for 2 other P. Farinosa pathotypes resistance (Pfs1 and Pfs 4)

10. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 25/12/2015 Evaluation for P1 : (WHRI)  What has been done : In 2007: evaluation of 28 lettuce accessions for 2 types of discoloration : browning and pinking (> 24 in the The analysis of the data has been carried out.  What will be done in 2009 : Data to be provided to lettuce database manager and WP coordinator

11. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 25/12/2015 Evaluation for P2 (GEVES)  What has been done : Evaluation of disease resistance : - 14 accessions to Sclerotinia sclerotiorum (FNPE): data on witlooves collected on Feb. 2009 - 17 accessions to Thielaviopsis basicola (2 nd test about to be effective in summer 09) - 22 accessions to Alternaria cichorii (data sent to database manager)  What will be done in 2009 : - data collected in Feb. 09 (S. Scleritiorum) to be provided to the chicory database manager - evaluation of disease resistance : 72 accessions to T. Basicola and 20 accessions to A. cichorii

12. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 25/12/2015 Evaluation for P4 (Palacky)  What has been done : - Selection of 30 lettuce accessions for characterization. - In 2008, evaluation of 30 lettuce accessions for response to 3 Bremia lactucae isolates ; 2008 data sent to the WP3 leader  What will be done in 2009 : - 2008 data to be formatted and sent to database manager - In 2009, evaluation of the lettuce accessions for response to 9 new isolates of Bremia lactucae - In 2010, depending on the exact 2009 B.l. Races tested, 8 races will be tested

13. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 25/12/2015 Evaluation for P5 (Nordgen)  What has been done : - Rucola (Eruca) : 20 accessions collected and submitted in 2008 to chemical evaluations (nitrate, oxalic acid) ; results sent to database manager. - Spinach : 51 accessions tested in 2008 (60 scheduled) to chemical evaluations with data sent to database manager.  What will be done in 2009 : Chemical evaluations of 16 orache (Atriplex hortensis) : spinach or minor crops ?

14. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 25/12/2015 Evaluation for P7 (Arche Noah)  What has been done : - Evaluation on winter hardiness made on 6 lettuce accessions planted in October 08. - Data to be provided to the database manager  What will be done in 2009 : - Evaluation for quality of 15 other lettuce accessions

15. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 25/12/2015 Evaluation for P8 (HDRA)  What has been done : Evaluation in 2008 in the field of 16 lettuce accessions (instead of 17) for Bremia lactucae  What will be done in 2009 : data for 2008 evaluation to be provided to the database manager

16. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 25/12/2015 Evaluation for P9 (University of Bologna)  What has been done : –Evaluation in 2008 of 37 Rucola accessions (Eruca sativa, Diplotaxis tenuifolia) for glucosinolates content, –Data have been sent to the database manager  What will be done in 2009 : - Evaluation for sesquiterpenes in chicory on 97 accessions (mainly leafy chicories) : - seeds of 75 accessions sent from P2 – GEVES for plant production during 2009 summer - seeds of 11 accessions already available in P9 place - production of roots is on going for 11 accessions for analysis on witlooves during first months of 2010

17. P Coquin : 3rd WP coordinators meeting Leafy Vegetables, Warwick, 11 June 2009 25/12/2015 Evaluation for P10 (KIS)  What has been done : Evaluation of 7 accessions for tolerance- resistance to root-knot nematodes (Meloïdogyne ethiopica).  What will be done in 2009 : - Transmission of data to WP3 coordinator and database manager - Evaluation of 7 other accessions for nematode resistance/tolerance.