1. CONTROL STRATEGY OF FMD IN LIBYA AND POST-VACCINATION MONITORING Dr. Ibrahim Eldaghayes Faculty of Veterinary Medicine University of Tripoli Tripoli, Libya ibrahim.eldaghayes@vetmed.edu.ly Abdunaser Dayhum; Abdulwahab Kammon; Monier Sharif; Giancarlo Ferrari; Keith Sumption; Donald King; Santina Grazioli and Emiliana Brocchi

2. Libya GFRA Meeting 20-22 October 20152

3. History of FMD in Libya: SerotypeYears FMDV-O:1959, 1962, 1967-1968, 1972, 1981-1983, 1988-1989, 1994, 2010-2013 FMDV-A:1979, 2009 FMDV-C:0000 FMDV-Asia 1:0000 FMDV-SAT 1:0000 FMDV-SAT 2:2003, 2012 FMDV-SAT 3:0000 Source: http://www.wrlfmd.org/http://www.wrlfmd.org/ GFRA Meeting 20-22 October 20153

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5. Genotyping GFRA Meeting 20-22 October 20155

6. EuFMD Real-Time Training Course in Kenya (Jan 2013) GFRA Meeting 20-22 October 20156

7. Real-Time Training Course Lectures in Libya GFRA Meeting 20-22 October 20157

8. FMD Scientific Day Tripoli, 30/03/2014 GFRA Meeting 20-22 October 20158

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11. GFRA Meeting 20-22 October 201511 Rapid Response Teams (RRT)

12. FMD lateral-flow devices: received from EuFMD GFRA Meeting 20-22 October 201512

13. GFRA Meeting 20-22 October 201513

14. The Progressive Control Pathway for FMD control GFRA Meeting 20-22 October 201514

15. Progressive Control Pathway (PCP) and Risk Based Strategic Plan implementation: Component 2.3 of EuFMD workplan (REMESA): Assist national FMD risk management as part of the REMESA action plan. WeekDate Week 126-30/01/2014 Week 223-27/03/2014 Week 3Postponed due to security reasons Week 4Postponed due to security reasons GFRA Meeting 20-22 October 201515

16. GFRA Meeting 20-22 October 201516

17. GFRA Meeting 20-22 October 201517

18. GFRA Meeting 20-22 October 201518

19. GFRA Meeting 20-22 October 201519

20. GFRA Meeting 20-22 October 201520

21. Mass Vaccination Campaign in Libya Sheep, Goats and Cattle: A & O Merial Vaccine Strains: O Manisa – O 3039 ; A Iran 05; (SAT-2 in 2012) Killed and purified vaccine 250 Vaccination Teams Once per year (2012 & 2013) 2014: Small ruminant once/year Cattle twice/year GFRA Meeting 20-22 October 201521

22. Vaccine Matching GFRA Meeting 20-22 October 201522

23. Agreement between the Libyan government and IZSLER, Italy 2012 GFRA Meeting 20-22 October 2015

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25. GFRA Meeting 20-22 October 201525

26. GFRA Meeting 20-22 October 201526

27.  Component 1 investigations in FMD suspected outbreaks.  Component 2 focused on the immune response of vaccinated animals.  Component 3 focused on serology as a mean to investigate on the level of FMD virus circulation (anti-NSP antibodies) and on the serotypes present (anti-SP serotype-specific antibodies). GFRA Meeting 20-22 October 201527

28. Component 1 (FMD suspected outbreaks – Clinical cases) GFRA Meeting 20-22 October 201528

29. COMPONENT 2 (immune response in vaccinated animals) This component has addressed the immune response of vaccinated animals. The outcome to be measured will be the level of antibodies before the vaccine is injected and the level of antibodies after 30 days from the date of vaccination. The animals (Cattle and small ruminants) selected for sampling were ear-tagged and blood samples were collected at the day of vaccination and repeated after 30 days. GFRA Meeting 20-22 October 201529

30. GFRA Meeting 20-22 October 201530

31. Component 2: Immune response in vaccinated animals Sampling and testing criteria Anti-NSP Ab and titration of SP Abs (serotypes O, A, SAT2)  before and 30 days PV For each species (goat, sheep, cattle): 150 animals, 6-12 month- old, ear-tagged Possibly, 10 different owners for each species (15 animals for each owner) GFRA Meeting 20-22 October 201531

32. Immune response to vaccine in small ruminants farms 7 FARMS Vaccine: type O+A ≈ 30 animals/farm 6 farms NSP-NEG 1 farm NSP-POS GFRA Meeting 20-22 October 201532

33. Immune response to vaccine in small ruminants 6 FARMS NSP neg Vaccine: type O+A ≈ 30 animals/farm SEROCONVERSION 30 DPV Serotype O 135/164 (82%) Serotype A 152/164 (93%) GFRA Meeting 20-22 October 201533

34. Immune response to vaccine in 1 cattle farm Seroconversion 30 DPV OASAT 2 N +30/30 29/30 % +100 % 97 % Vaccine: types O+A+SAT2 30 cattle, NSP neg GFRA Meeting 20-22 October 201534

35. Current work Jan 2015 – Jan 2016 Cattle: Collection of serum from unvaccinated animals at the time of injection of the vaccine (time 0) and tag the animals from which the sample has been collected (n=99). Collect serum after 2, 4 and 6 months. Booster vaccination at 6 months for 70 animals. Collect serum again after 2, 4 and 6 months. GFRA Meeting 20-22 October 201535

36. EuFMD Team (March 2015) GFRA Meeting 20-22 October 201536

37.  Thanks to: EuFMD IZSLER, Brescia and Ministry of Health, Italian Government FAO OIE REMESA Pirbright Lab GFRA Meeting 20-22 October 201537

38. GFRA Meeting 20-22 October 201538