1. Visualization for 3D Microscope Data Jonathan Robbins

2. Purpose Provide a tool to assist scientists visualize 3-D dataProvide a tool to assist scientists visualize 3-D data

3. Motivating Problems Cystic Fibrosis How does the mucus layer interact with the cilia?

4. Motivating Problems Where do proteins attach to Dendritic Spines?

5. Motivating Problems Imaging Kinetochores During Mitosis

6. Current Visualization View each slice separatelyView each slice separately View 3-D visualizations from fixed camera positionsView 3-D visualizations from fixed camera positions

7. Our Software Enables user to adjust camera position arbitrarily and in real- timeEnables user to adjust camera position arbitrarily and in real- time User can switch among various visualization techniquesUser can switch among various visualization techniques Rendering done by VTKRendering done by VTK Interface written with Java SwingInterface written with Java Swing

8. VTK Visualization Toolkit developed by Kitware, Inc.Visualization Toolkit developed by Kitware, Inc. Open-source 3-D data visualization softwareOpen-source 3-D data visualization software Written in C++Written in C++ Wrappers in Java, Python, and Tcl.Wrappers in Java, Python, and Tcl.

9. Demo

10. Where do we go from here? Extensions to VTKExtensions to VTK –12-bit TIFF files –Read file types not currently supported by VTK –Support Volume rendering of multiple –datasets Improved techniques for selecting isovalues and transfer functionImproved techniques for selecting isovalues and transfer function –Would like to be able to select a feature and have the isovalue and transfer function automatically set to display similar features