1. Zebrafish Project !!! Use the zebrafish set up genetic experiment
study embryonic development

2. Facts about Zebrafish Habitat- India, and Burma. In Ganges River
Life span 2-4 years
Grows to approximately 1 ½ inches
Diet- brine shrimp, smaller fish, insect larvae
Predators- larger fish, birds, snakes, frogs
Able to regenerate fins and other body parts

3. Why use zebrafish in research? (a good Model Organism)
Embryos are see through (can see organs and cells)
Have similar organs as humans
Inexpensive - $1.00 per fish
Easy to care for
Produce lots of offspring
Short lifespan (2 years)

4. Each Group
Fill the breeding tanks 1/2 full with water from the containers set up by the tanks (Do not use tap water)
Use nets to get 1 male and 1 female zebrafish and place the fish in your breeding tank
Label your tank with tape:
-Your names
-Names of your fish
-Phenotype of your fish (striped or albino)
-Number of tanks that you got the male and female from

5. Male and Female

6. Scientific Problem
The pigmentation that offspring have depends on the genes passed down from their parents.
What will the offspring of your zebrafish cross look like?
Take a few minutes to discuss with your partner. Draw a Punnett square to justify your hypothesis and include it in your manual.

7. Striped and Albino

8. Your Investigation!!
Cross fish and observe what the offspring look like
Record cross in your lab manual
Observe the embryonic development throughout the week