1. Automated Maze System Development Group 9 Tanvir Haque Sidd Murthy Samar Shah Advisors: Dr. Herbert Y. Meltzer, Psychiatry Dr. Paul King, Biomedical Engineering

2. Introduction  Microdialysis Method of measuring physiological activity during task Dr. Meltzer’s Lab uses it to study brain activity during memory tasks

3. Experimental Setup  Rat hooked up to Microdialysis  Rat placed in Maze, performs memory tasks  Sample collected during maze run  Sample Analyzed for content

4. Problems  Dialysis tubes’ entanglement  Rat’s recognition of overhead device  psychological repercussions  Manual guiding of tubes cumbersome for researcher

5. Constraints  Maze Dimensions  Rat Size  Rat Speed  Rat Cognition  Tube Length  Dialysis Weight Depth: 18”

6. Primary Objective  To develop a fully independent research module that facilitates the study of memory.

7. System Description Acquire Mouse Position Determine Change in Position Translate Dialysis Machine

8. Position Acquisition MethodProsCons Camera  High Resolution  Real-Time feedback  Software intensive  Mounting Issues  Processing Limitations Sensor  Manageable data  Less processing  Low resolution  Center of maze difficult to map

9. Image Processing Acquire ImageCalibrate the Image Convert the 32 bit image to an 8 bit image Filter Image 1: Remove Border Objects Filter Image 2: Remove Small Objects Pattern Match to a Specified Image Determine the pixel at the center of the pattern Translate pixel value into physical coordinates Output Physical coordinates in array form

10. LabView Software Code

11. Image Processing Unprocessed ImageProcessed Image

12. LabView Screen Shot

13. Choosing a Microprocessor Motorola 68HC11E  One 8-bit input  Low cost  On board A to D converter NI PCI-7342  Four 8-bit inputs  More processing capabilities  Software Compatibility with LabView

14. Processing the Information  Continually Given one set of coordinates (X,Y)  Compares the coordinates of (X n-1,Y n-1 ) to (X n,Y n ), computes the difference, and rounds the significant digits  Converts the difference into specified timed waveform for the driver  Driver amplifies signal and controls motor speeds

15. Drive System  Lead-screw Device Relatively Easy to build Not very efficient Cheap  Pulley/Belt System Complicated System Efficient Expensive

16. The Lead-Screw Device Motor Driven Rotational Energy converted to Linear Energy

17. Device Apparatus

19. Choosing a Motor  Design Considerations: Speed of Mouse: roughly 2 ft/s Torque  Torque needed to drive apparatus  Torque needed to provide acceleration  Stepper Motor or DC Motor?

20. Speed Lead (in/rev)RPM.12511520.255760.52880 RPM = 25.5 in/s / Lead*60 s/min Target RPM Range 3000 -12000

21. Torque  Driving Torque  Acceleration  Driving Torque 2 ft/s L = 2.37 lbs P =.5 in/rev e f =.4 (for ACME) T f = 53 mNm Position Time 25 in -25 in I = 0.001207 lb-in-s 2 α = 265 rad/s 2 T = 36 mNm Worst Case Scenario

22. Stepper or DC?  Stepper Torque < 3.53 Nm RPM < 2000  DC High Torque High RPM

23. DC Motor  3000 RPM (using 0.5 lead)  87 mNm Torque  Powered by Driver  Monitored by external Optical Encoder

24. Flow Chart Image Calculate Δ(x,y) Micro- Processor Driver Motor Translation Image Calculate Δ(x,y) Micro- Processor Driver Motor Translation

25. Budget Support Scaffolding$99.70 Mechanical Arm (including driver electronics) $1294.84 Microcontroller$895.00 Labview/Imaging Software$805.00 Grand Total$3094.54

26. Overall Status  Project on Schedule  Parts and budget completed  Next step: Offer proposal for funds and order parts Month/ TasksJanuaryFebruaryMarchApril Breakdown of parts needed Researched parts and obtained quotes Offer proposal for parts and order Build Scaffolding system and check operation Test and refine LabView software Trials with software and microcontroller Trials with software, microcontroller and hardware Test / Debug the entire system

27. Conclusion  The device designed will automatically determine the position of the rat and move the dialysis machine accordingly with minimal slack of the tubing.