1. A B C 3500x8900x 5600x14 000x 1500x5000x Supplementary Figure 1 TiO 2 Carbon black SWCNT

2. Supplementary Figure 2 DMEM P-IκBα DAPI Merge SWCNT WT p53 KO WTp53 KO P-2κBα immunostaining of WT and p53 KO macrophages Immunostaining quantification Percentage of positively stained cells (%) 2µg/cm 2 A B

3. Supplementary figure legends Supp Fig 1. Transmission electron microscopy images of RAW 264.7 cells incubated with 10 µg/ml (2 µg/cm 2 ) of manufactured nanomaterials (MNMs) for 6 hr. (A) RAW 264.7 cell with TiO 2 A10 MNMs within a vesicle (B) CB P60 MNMs within the cytosolic compartment of a RAW cell. (C) RAW macrophages with SWCNT at the membrane surface. Supp Fig 2. Consequence of p53 activation: inflammatory response in primary wild-type (WT) and p53 knock-out (KO) macrophages. (A, B) The cells were exposed to 2 µg/cm 2 of TiO 2 (A10), CB (FW2) and SWCNT MNMs for 30 min. The data show the percentages of phosphorylated IκBα (P- IκBα) positively stained macrophages (mean ± SEM) (n=3). * significant difference from WT control (p < 0.05). # significant difference between WT and p53 KO macrophages (p < 0.05).