1. Proteomics: 2D Gel

2. Proteome analysis 장치 (2D)

3. Root ProteomeNodule Proteome

4. Table I. Proteins identified in soybean root and nodule through MALDI-TOF/MS & LC-MS/MS Spot No. Calc. pI/ Mw a Z-score b / Mascot score c Identify d Species (NCBI accession No.) Theor. pI/MW SC e (%) Status Analy -zer f Ⅰ. Primary metabolism sr038 g 6.19/411.65/ - Cytosolic malate dehydrogenase Cicer arietinum (CAC10208) 5.9/35.8224.m sr1505.98/420.99/ - Malate dehydrogenase, cytoplasmic Beta vulgaris (Q9SML8) 5.9/35.82 205d-upm sr1515.98/421.1/ - Malate dehydrogenase, cytoplasmic Beta vulgaris (Q9SML8) 5.9/35.82205d-upm sr1606.42/410.85/ - Malate dehydrogenase, Mdh-2, mitochondrial Glycine max (T06326) 5.6/27.5415.m sr1616.42/411.6/ - Malate dehydrogenase, Mdh-2, mitochondrial Glycine max (T06326) 5.6/27.5421.m sn125 h 6.41/38-/ 741Malate dehydrogenase Bradyrhizobium japonicum USDA 110 (NP_767096) 5.88/34.144.72 10d-new, 13d-up t ssn0026.00/43-/ 62Malate dehydrogenase Glycine max (AAD56659) 8.23/36.18.1110d-newt sr1015.40/400.23/ - pfkB-type carbohydrate kinase family protein Arabidopsis thaliana (NP_180697) 5.3/35.4318.m sr1125.30/400.89/ - pfkB-type carbohydrate kinase family protein Arabidopsis thaliana (NP_180697) 5.3/35.4324.m

5. 단백질의 질량 스펙트럼 단백질 효소 질량분석기 MS spectrum Peptide sequences Protein database Protein id A B AB

6. Proteomics: MALDI-Mass

7. Mass spectrum vs. database Mass spectrumdatabase Protein A Protein B Protein C

8. 탄뎀 질량 스펙트럼 (MS/MS spectrum) 단백질 효소 1st MS 2nd MS MS spectrum energy Tandem MS spectrum Peptide sequence Protein database Protein id

9. 탄뎀 질량 스펙트럼 Peptide fragmentation H2NH2N COOH R1 C H O N H C R2 C H O N H C R3 C H O N H C R4 C H x 3 y 3 z 3 x 2 y 2 z 2 x 1 y 1 z 1 a 1 b 1 c 1 a 2 b 2 c 2 a 3 b 3 c 3

10. 2D data 분석