1. A&P 120 Enzyme Function Exercise 4 www.celltech.com/resources/vt/enzymes.html

2. Terminology Metabolism –Definition: The sum total of all biochemical activity that takes place in a living organism Catabolic Metabolism – break down –AB = A & B Anabolic Metabolism – build up –A + B = AB In vitro – out of the body (or in a tube) –In Vivo – in the body Substrate - The material being acted upon

3. Introduction Enzyme – a protein that is a organic catalyst –Hint: Most of the time ends in –ase Catalyst – speeds up the rate of a reaction –Catalase – is the name of an enzyme in your body Active Site – a pocket at the outside of the enzyme specific to a substrate (here is where it is taken apart or put together) 2H 2 O 2 2H 2 O +O 2 Catalase (organic) (Hydrogen peroxide) Substrate (Water & Oxygen) Product **Oxygen will bubble in vitro** Bubbles Catalase H2O2H2O2 H2O2H2O2

4. Temperature Denature: enzyme unravels/unfolds and loses structure and function COLDHOT Low activity High activity -cold temperatures inhibit (prevent) enzyme activity -hot temperatures denature enzymes Best temperature Normal enzyme Extreme heat Denature d enzyme p. 35?

5. pH Acidic –less than 7 Basic (alkaline) – greater than 7 Neutral = 7 Enzymes have a certain pH at which they work best -above or below optimum pH inhibits activity pH = 0pH = 14 p. 35?

6. How Enzymes Work Biological Catalysis (usually proteins) – Make a reaction faster Enzymes are Proteins – built to work “active site” Proteins are Happy’ist in their natural Temp and pH –Usually ~37 0 C or 98 0 F & Usually a neutral pH (Function) –What would happen if you boil it? Guesses -___________ –What would happen if you put it in acid? ________________ –What would happen in the cold?________________ Enzymes can only work so fast…….. –If you keep adding substrate will the speed of the Rxn Catalase H2O2H2O2 O 2 & H 2 O Catalase or a)b) Poison What does cyanide do? Arsenic?

7. How Enzymes Work Biological Catalysis (usually proteins) – Make a reaction faster Enzymes are Proteins – built to work “active site” Proteins are Happy’ist in their natural Temp and pH –Usually ~37 0 C or 98 0 F & Usually a neutral pH (Function) –What would happen if you boil it? Guesses -____denature________ –What would happen if you put it in acid? _______denature_________ –What would happen in the cold?________inhibit________ Enzymes can only work so fast…….. –If you keep adding substrate will the speed of the Rxn Catalase H2O2H2O2 O 2 & H 2 O Catalase or a)b) Poison What does cyanide do? Arsenic?