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In Vivo Optical Imaging: Human Application?

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Presentation on theme: "In Vivo Optical Imaging: Human Application?"— Presentation transcript:

1 In Vivo Optical Imaging: Human Application?
Vassiliy Tsytsarev University of Maryland school of Medicine In this study, optical-resolution photoacoustic microscopy was used to monitor the somatosensory cortex for vascular responses to direct electrical stimulations through a cranial window.

2 Glucose Metabolism Capillary Glycogen TCA TCA
Yongmei Chen and Raymond A Swanson Journal of Cerebral Blood Flow & Metabolism (2003) 23, 137–149; Astrocytes and Brain Injury

3 Glucose Fluorescence Substitute: 2-NBDG
2-deoxy-d-glucose 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-d-glucopyranose (2-NBDG) D-glucose

4 Glucose Fluorescence Substitute: In vivo imaging of epileptic activity using 2-NBDG

5 Epileptic Seizures: Fluorescence Imaging

6 fMRI vs NIRS

7 Light Banana

8 Light Banana: Oxygenation

9 Main parts of experimental setup

10 HITACHI: medical application

11 Spectrum: T-ray

12 EROS: Optical correlation of the EEG?
Evoked Response Optical Signal (EROS) (Gratton et al, 2002, 6, 7)

13 T-ray imaging

14 Acknowledgments Drs. Shigeru Tanaka, Hidenao Fukuyama, Kazuyuki Imamura, Ayako Ajima, Hisayuki Ojima, Minoru Kimura and Jerom Ribot – Brain Science Institute of RIKEN and Kyoto University, Japan Dr. Sonya Bahar, Director, Center for Neurodynamics, University of Missouri at St. Louis Daisuke Takeshita and Douglas Joseph Brumm – Ph.D students, Center for Neurodynamics, University of Missouri at St. Louis Song Hu, Junjie Yao, Li Li – Ph.D. student of the Washington University in St. Louis Drs Konstantin Maslov and Lihong Wang – Department of Biomedical Engineering, Washington University in St. Louis

15 Thank you very much for your attention


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