Presentation is loading. Please wait.

Presentation is loading. Please wait.

Rapid biodiversity discovery in megadiverse tropical systems using Next Generation Sequencing (NGS) barcodes Darren YEO 1, ​Amrita Srivathsan 1, Wendy.

Published byPiers Phillips Modified over 9 years ago

Similar presentations

Presentation on theme: "Rapid biodiversity discovery in megadiverse tropical systems using Next Generation Sequencing (NGS) barcodes Darren YEO 1, ​Amrita Srivathsan 1, Wendy."— Presentation transcript:

1 Rapid biodiversity discovery in megadiverse tropical systems using Next Generation Sequencing (NGS) barcodes Darren YEO 1, ​Amrita Srivathsan 1, Wendy Wang 1, Maosheng Foo 2, Jayanthi Puniamoorthy 3, Wong Wing Hing 1, Rudolf Meier 1 1 Department of Biological Sciences, National University of Singapore 2 Lee Kong Chian Natural History Museum, National University of Singapore 3 California Academy of Sciences, San Francisco State University Images from Animals & Plants of Singapore

2 What do we know about our invertebrate biodiversity? Butterflies: ~290 spp Dragonflies: ~128 spp Crabs: >350 spp Spiders: >310 spp True Flies: ? spp Beetles: ? spp Nematodes: ??? spp Mites: ??? spp 50 000 spp?

3 Next Generation Sequencing Barcoding Direct PCR of COI gene Sample pooling with labelled primers Illumina Mi/HiSeq high throughput sequencing Cost: Sanger barcoding: USD 10.00 – 30.00 per specimen NGS barcoding: USD 0.50 – 0.57 per specimen

4 Fungus Gnats (Mycetophilidae) 1201 Specimens barcoded Ca. 215 Species/OTUs

5 Soldier Flies (Stratiomyidae) 418 Specimens barcoded Ca. 31 Species/OTUs

6 Termites (Termitoidae) 363 Specimens barcoded Ca. 41 Species/OTUs

7 Rapid Species Discovery Fungus Gnats - 1201 specimens barcoded - ca. 215 Species/OTUs Mosquitos - 2163 specimens barcoded - ca. 95 Species/OTUs Ants - 1904 specimens barcoded - ca. 100 Species/OTUs Termites - 363 specimens barcoded - ca. 41 Species/OTUs Bees & Wasps - 489 specimens barcoded - ca. 84 Species/OTUs Soldier Flies - 418 specimens barcoded - ca. 31 Species/OTUs Hoverflies - 266 specimens barcoded - ca. 23 Species/OTUs Horseflies - 363 specimens barcoded - ca. 25 Species/OTUs 7167 Specimens Barcoded Ca. 614 Species/OTUs Span of half a year

8 Abundance & Distribution Information

9 Life History Stage Matching

10 Acknowledgements P. I. Prof. Rudolf Meier Co-authors Dr. Amrita Srivathsan, Dr. Wendy Wang, Dr. Ang Yuchen, Mr. Foo Maosheng, Ms. Jayanthi Puniamoorthy, Mr. Wong Wing Hing Other members and students of the NUS Evolution Lab Interns from Republic Polytechnic

Download ppt "Rapid biodiversity discovery in megadiverse tropical systems using Next Generation Sequencing (NGS) barcodes Darren YEO 1, ​Amrita Srivathsan 1, Wendy."

Similar presentations

Next-Generation Sequencing: Methodology and Application

Next-Generation Sequencing: Methodology and Application
contents Main cover 1 Main cover 1 Dragonflies 4 Dragonflies 4 Grasshopper 5 Grasshopper 5 Ladybirds 6 Ladybirds 6 Ants 7 Ants 7 Beetles 8 Beetles 8 Bees.

contents Main cover 1 Main cover 1 Dragonflies 4 Dragonflies 4 Grasshopper 5 Grasshopper 5 Ladybirds 6 Ladybirds 6 Ants 7 Ants 7 Beetles 8 Beetles 8 Bees.
Insects Ms Shader.

Insects Ms Shader.
Animals There are 2 groups:

Animals There are 2 groups:
From Bugs to Barcodes: Using Molecular Tools to Study Biodiversity Mandy Butler, Heather Henter, Stephanie Mel University of California, San Diego.

From Bugs to Barcodes: Using Molecular Tools to Study Biodiversity Mandy Butler, Heather Henter, Stephanie Mel University of California, San Diego.
Welcome to “Bug” Week Test scores, not terrible Snack Bar reminder Bug Day is Friday! Use the laptops to get vocabulary and chart done Lab (sketches) and.

Welcome to “Bug” Week Test scores, not terrible Snack Bar reminder Bug Day is Friday! Use the laptops to get vocabulary and chart done Lab (sketches) and.
Life Cycles.

Life Cycles.
Major Orders of Insects. 1. Grasshopper, cricket, roach, mantid 2. true bug 3. cicadas or leaf or plant hopper 4. beetle 5. fly 6. ant, bee or wasp 7.

Major Orders of Insects. 1. Grasshopper, cricket, roach, mantid 2. true bug 3. cicadas or leaf or plant hopper 4. beetle 5. fly 6. ant, bee or wasp 7.
Introduction to Insects. Outline Insects and their relatives How insects rule the world Insect anatomy and biology.

Introduction to Insects. Outline Insects and their relatives How insects rule the world Insect anatomy and biology.
Insects Block 1 January 13, 2015.

Insects Block 1 January 13, 2015.
A Bug’s Life.

A Bug’s Life.
Origins & Evolution: informatics activities and needs Steve Brooks Natural History Museum London, UK.

Origins & Evolution: informatics activities and needs Steve Brooks Natural History Museum London, UK.
Insects © 2008 Quinín Freire. There are more than1,000,000 species of insects in Nature.

Insects © 2008 Quinín Freire. There are more than1,000,000 species of insects in Nature.
This is the presentation Ms. Rieke used to introduce Invertebrates to her students.

This is the presentation Ms. Rieke used to introduce Invertebrates to her students.
Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes Fulgerator Paul Hebert, Erin Penton, John Burns,

Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes Fulgerator Paul Hebert, Erin Penton, John Burns,
Using museum specimens to identify MOTUs in larval scarab beetles Andrew Mitchell, NSW DPI Kelly Rigg, Charles Sturt University Gus Campbell, NSW DPI Tom.

Using museum specimens to identify MOTUs in larval scarab beetles Andrew Mitchell, NSW DPI Kelly Rigg, Charles Sturt University Gus Campbell, NSW DPI Tom.
Insects By Bradley and Ernan. Bees - 2 Flies - 3 Ladybirds – 4 Ants – 5 Dragonflies – 6 Glossary - 7.

Insects By Bradley and Ernan. Bees - 2 Flies - 3 Ladybirds – 4 Ants – 5 Dragonflies – 6 Glossary - 7.
Minibeast Creature.

Minibeast Creature.
Molecular Biology Dr. Chaim Wachtel April 4, 2013.

Molecular Biology Dr. Chaim Wachtel April 4, 2013.
BioBarcode: a general DNA barcoding database and server platform for Asian biodiversity resources Jeongheui Lim Korean BioInformation Center Korea Research.

BioBarcode: a general DNA barcoding database and server platform for Asian biodiversity resources Jeongheui Lim Korean BioInformation Center Korea Research.

Similar presentations

Ads by Google