1. What is lactose intolerance?

2. Inability to digest lactose!

3. Catalyzed reaction Enzyme: Substrate (reactant): Products:

4. Lactose Intolerance lab  In pairs, you will observe what happens when you add lactase (enzyme) to regular milk and lactose- free milk.  You will have to figure out which milk is which!

5. Talk to the text  Plant-Based Ice Cream Hits European Markets  Science has given ice cream a makeover, expanding the number of options for people with lactose intolerance who cannot digest most dairy products.

6. LACTOSE INTOLERANCE LAB

7. Review TTTT  What was special about the ice cream “Lupinesse”?

8. Lactase catalyzed reaction

9. Steps  Have the partner on the left grab a green tray. ON THE YELLOW SHEET  Read the introduction and answer the pre-lab questions to prepare for the lab.  After you have answered all the questions, follow the experimental procedures.

10. Glucose indicator test  The glucose strips will tell you whether glucose is present in the liquid.  Dip the glucose strips in briefly so that the end gets wet with the liquid. Do not leave it in the liquid.  Wait 30 seconds and then check your results (color change) against the key provided.  Record both the color (in words) and the glucose concentration (in numbers).

11. Some tips!  The substances you will use are along the back counter.  Positive (+) control = glucose solution  Negative (-) control = water  A & B milk  Take your test tubes to the counter to get the substances.  Use the markings on your pipet to make accurate measurements.  Pipet = the tool like a dropper  Use the marker to crush the tablet WHILE IT IS STILL IN THE PACKET. Be careful not to puncture the packet.  Regular milk and lactose-free milk look the same, so make sure to label your test tubes.  Record your results as you go along.