1. OPTOFLUIDIC DEVICES FOR SINGLE CELL MANIPULATION Ana Rita Ribeiro, Ariel Guerreiro, Pedro Jorge New Challenges in the European Area - Young Scientist's 1st International Baku Forum 23-05-2013

2. Outline Introduction Main Principles Computational Model and Results Experimental Preliminary Results Conclusions and Future Work 23-05-2013Young Scientist's 1st International Baku Forum2

3. What are optical tweezers? 23-05-2013Young Scientist's 1st International Baku Forum3 Motivation Manipulation: Cell/particle manipulation withouth physical contact; Miniaturization: Diagnostic devices (in vivo cells,...) Main Goals Development of fiber optical tweezers; Study of the forces: Numerical models; Design and optimization of fiber structures; Fabrication of devices; Field of application: Manipulation of Red Blood Cells. Liu, Z. et al Opt. Express, OSA, 2006, 14, 12510-12516

4. Main Principles 23-05-2013Young Scientist's 1st International Baku Forum4 Ashkin, A., Dziedzic, J. M., Bjorkholm, J.E. and Chu, S. (1986), Opt Lett., 11, 288-290; Ashkin, A. (1970), Phys. Rev. Lett., 24, 156-159. Particle placed in the focusing region of a laser beam: Force  transference of momentum on the particle surface (reflections/refractions). Scatering Force Gradient Force

5. Computational model and Results 23-05-2013Young Scientist's 1st International Baku Forum5 Lorentz Force (dipoles): R. S. RODRIGUES RIBEIRO et al, “Computational Models for New Fiber Optic Tweezers”, Photonic Sensors

6. Results 23-05-2013Young Scientist's 1st International Baku Forum6

7. Preliminary Results Lens – polimer: – radius ~ 30-40 μm; Laser Diode 980 nm, P = 100mW; Polystyrene particles: 23-05-2013Young Scientist's 1st International Baku Forum7 Experimental Preliminary Results R. S. Rodrigues Ribeiro, et al., New theoretical and experimental methods for the design of fiber optic tweezers, EWOFS, Poland,2013

8. Conclusion and Future Work Development of a 2D numerical model to calculate optical forces acting on a particle/cell; Fabrication of lenses in optical fibers; Implementation of a 3D numerical model; Study of distinct fiber geometries; Manipulation of RBC. 23-05-2013Young Scientist's 1st International Baku Forum8

9. Thanks! 23-05-2013 Young Scientist's 1st International Baku Forum9