1. A New Test for Prostate Cancer Monthly Oral Presentation #3 By: Maja Zavaljevski and Alissa Schaeffer March 24, 2000

2. Prostate Cancer Statistics

3. About Prostate Cancer l General Information about Prostate Cancer l Some Symptoms l Molecular Biology of Cancer

4. Why is Prostate Cancer So Difficult to Diagnose? l Slow growing process 90% of prostate cancers remain dormant for decades l Other conditions show same symptoms Benign Prostatic Hyperplasia(BPH) Prostatitis Prostatosis

5. BPH vs Prostate Cancer

6. Disadvantages and Difficulties In Current Methods l DRE indicates only about 40% of possible tumors l PSA testing presents false negatives as well as false positives 25% of tumors go undetected 67% of high PSA’s show no cancer l Biopsy is an invasive procedure

7. Objectives l Design a device to diagnose prostate cancer in the early stages l Improve upon similar current methods that use molecular biology l Determine stage of disease

8. Insulin-like Growth Factors and Prostate Cancer l How the IGF system works l IGF Binding Protein II (IGFBP-2) Plasma Concentrations

9. Overview of Project l Testing for IGFBP-2 and PSA Two antigen ELISA test Flow Cytometry l Advantages of using this method

10. Conducting the Test l Two antigen ELISA (PSA and IGFBP-2) Solid Substrate - polystyrene beads in two sizes Fluorescent dyes - FITC and PE

11. Analyzing the Results l Flow Cytometry Generating calibration curve from standards Measuring absorbencies of the sample Read concentration from calibration curve

12. References and Thanks l Dr. David Kelso at Northwestern University l Dr. Todd Giorgio l Todd Monroe l Dr. Paul King