1. Ch. 13 Warm up 1. Compare sexual to asexual reproduction. 2. Compare/contrast mitosis to meiosis. 3. What are homologous chromosomes? 4. Describe what major processes occur during a sexual life cycle.

2. Ch. 13 Warm up 1. Describe and compare the 3 sexual life cycles 1. How does random assortment, crossing over and random fertilization contribute to genetic variation? 2. Define: locus, karyotype, alternation of generations, synapsis, tetrad, chiasmata

3. Warm up 1. Describe what occurs during crossing over. 2. What are 3 sources of genetic variation? 3. Mitosis, Meiosis, or Both? A. Chromosomes line up at metaphase plate B. Crossing over C. Cytokinesis D. Chromosomes are replicated E. Four haploid daughter cells result F. Two diploid daughter cells result

4. Chapter 13: Meiosis & Sexual Life Cycles

5. What you must know The difference between asexual and sexual reproduction. The role of meiosis and fertilization in sexually reproducing organisms. The importance of homologous chromosomes to meiosis. How the chromosome number is reduced from diploid to haploid through the stages of meiosis. Three important differences between mitosis and meiosis. The importance of crossing over, independent assortment, and random fertilization to increasing genetic variability.

6. Types of Reproduction ASEXUAL Produces clones (genetically identical) Single parent Little variation in population - only through mutations Fast and energy efficient Eg. budding, binary fission SEXUAL Meiosis produces gametes (sex cells) 2 parents: male/female Lots of variation/diversity Slower and energy consumptive Eg. humans, trees

7. Asexual vs. sexual reproduction

8. Genes: segments of DNA that code for basic units of heredity Offspring acquire genes from parents by inheriting chromosomes

9. Chromosomes Somatic (body) cell: 2n = 46 chromosomes Each pair of homologous chromosomes includes 1 chromosome from each parent Autosomes: 22 pairs of chromosomes that do not determine sex Sex chromosomes: X and Y Females: XX Males: XY Gametes (n=23): 22 autosomes + 1 sex chromosome Egg: 22 + X Sperm: 22 + X **or** 22 + Y

10. Homologous Chromosomes in a Somatic Cell

11. Meiosis = reduction division twice Cells divide twice Result: 4 daughter cells, each with half as many chromosomes as parent cell, each daughter cell is genetically unique

12. Meiosis I Meiosis I (1 st division) Interphase: chromosomes replicated Prophase I: Synapsis: homologous chromosomes pair up Tetrad = 4 sister chromatids Crossing over at the chiasmata Metaphase I: Tetrads line up Anaphase I: Pairs of homologous chromosomes separate (Sister chromatids still attached by centromere) Telophase I & Cytokinesis: Haploid set of chromosomes in each cell Each chromosome = 2 sister chromatids Some species: chromatin & nucleus reforms

14. Meiosis II Meiosis II (2nd division) = create gametes Prophase II: No interphase No crossing over Spindle forms Metaphase II: Chromosomes line up Anaphase II: Sister chromatids separate Telophase II: 4 haploid cells Nuclei reappear Each daughter cell genetically unique

16. Events Unique to Meiosis I (not in mitosis) 1. Prophase I: Synapsis and crossing over 2. Metaphase I: pairs of homologous chromosomes line up on metaphase plate 3. Anaphase I: homologous pairs separate  sister chromatids still attached at centromere

17. Sources of Genetic Variation: 1. Crossing Over Exchange genetic material Recombinant chromosomes

18. Sources of Genetic Variation: 2. Independent Assortment of Chromosomes Random orientation of homologous pairs in Metaphase I

19. Sources of Genetic Variation: 3. Random Fertilization Any sperm + Any egg 8 million X 8 million = 64 trillion combinations!

20. MitosisMeiosis Both are divisions of cell nucleus Somatic cells 1 division 2 diploid daughter cells Clones From zygote to death Purpose: growth and repair No synapsis, crossing over Gametes 2 divisions 4 haploid daughter cells Genetically different-less than 1 in 8 million alike Females before birth follicles are formed. Mature ova released beginning puberty Purpose: Reproduction

22. Mitosis vs. Meiosis

23. Life cycle: reproductive history of organism, from conception  production of own offspring Fertilization and meiosis alternate in sexual life cycles Meiosis: cell division that reduces # of chromosomes (2n  n), creates gametes Fertilization: combine gametes (sperm + egg) Fertilized egg = zygote (2n) Zygote divides by mitosis to make multicellular diploid organism

24. Varieties of Sexual Life Cycles

25. Human Life Cycle

26. Animals

27. Plant and some algae Sporophyte (2n): makes haploid spores by meiosis Spore  gametophyte by mitosis Gametophyte (n): makes haploid gametes by mitosis Alternation of Generations

28. Fungi, protists, algae

29. Karyotype: a picture of an organism’s complete set of chromosomes Arranged from largest  smallest pair

30. Making a karyotype – unsorted chromosomes

31. 22 pairs of autosomes + 1 pair of sex chromosomes Male or female?

33. Karyotype - used to determine genetic abnormalities

34. Cancer cells Some have abnormal #’s of chromosomes Karyotype of Metastatic Melanoma

35. Breast Cancer Cell Karyotype

36. HeLa Cells Oldest and most commonly used human cell line Cervical cancer cells taken from Henrietta Lacks (d.1951)

37. HeLa Cells “Immortal” cells – do not die after a few divisions Active version of telomerase Used in research: Develop vaccine for polio Cancer, AIDS, virus, radiation research Estimated that cells produced in culture exceeded # cells in Henrietta’s body

38. HeLa Cell Karyotype

40. HeLa Cells – Ethical Concerns Controversy: Cells harvested without patient consent “Discarded tissues can be commercialized” – sold for profit Genome published in 2013 without family’s consent “The Immortal Life of Henrietta Lacks” by Rebecca Skloot

41. Check for understanding 1. What phase directly follows metaphase I? 2. How many cells are present at the end of meiosis I ? 3. A cell has a diploid number of 60, what is the organism's haploid number? 4. Meiosis occurs in what type of cells? 5. In what phase do homologous chromosomes pair up and crossing-over can occur? 6. In what phase do the CHROMATIDS separate? anaphase I 2 30 gametes prophase 1 anaphase 2

42. 7. Identify the phase:8. Identify the phase: anaphase 1 anaphase 2

43. Concept Check 1. Meiosis results in _____ a. 2 haploid daughter cells b. 4 haploid daughter cells c. 2 diploid daughter cells d. 4 diploid daughter cells 2. Which of the following cells undergo meiosis? a. sperm cells b. liver cells c. unicellular organisms d. all of these 3. The picture depicts what phase of meiosis a. prophase 1 b. prophase 2 c. anaphase 1 d. anaphase 2

44. Concept Check 1. Crossing-over occurs during: a. anaphase 1 b. metaphase 1 c. prophase 1 d. prophase 2 2. A cell with a diploid number of 24 undergoes meiosis, how many chromosomes are in each daughter cell? a. 6 b. 12 c. 24 d. 48

45. Concept Check

46. Name of PhaseDescription 1. Homologous chromosomes pair up and form tetrad 2. Spindle fibers move homologous chromosomes to opposite sides 3. Nuclear membrane reforms, cytoplasm divides, 4 daughter cells formed 4. Chromosomes line up along equator, not in homologous pairs 5. Crossing-over occurs 6. Chromatids separate 7. Homologs line up alone equator 8. Cytoplasm divides, 2 daughter cells are formed