1. P1-1 Cell diagram 3P 1-1

2. Chromatin to DNA From chromosome to chromatin to solenoid to DNA

3. P3-2 Chromosome to DNA 3P3-2 Chromosomes

4. P3-2 Backbone chemistry 3P3-2 1 23 4 5 O 5 3

5. 4F4-4

6. 3F2-10 4F4-3

7. 8-30 chromatin 3F8-30

8. 4F4-21 2 linked copies of a chromosome during mitosis Tangle of chromatin spilling out of lysed nucleus

9. Central Dogma Transcription mRNA Gene (DNA) Translation Protein Transcription Reverse Transcription

11. exons

13. 27Kbp 300Kbp Gene Ex1Ex2 Intron1 Intron2 145bp 2500bp 5 ’ UTR 3 ’ UTR JUNK???

14. 3-12,13 DNA replication 4F5-5 DNA Synthesis Template, 3’ 5’ New, 5’ 3’ 4F5-3

15. CELL CYCLE: Gap1, Synthesis, Gap2, Mitosis ….

17. proteins- chain of AA O -- C NH3+NH3+ H C R1R1 O C NHNH H C R2R2 O C NHNH H C R3R3 O C NHNH H C R4R4 O R1R1 R2R2 R1R1 R2R2 Peptide bond

18. CRAMBIN: N = 46 TTCCPSIVARSNFNVCRLPGTPEALCATYTGCIIIPGATCPGDYAN Peptide bond,crambin

19. 3-35 3-35 primary to quaternary

20. 4F6-8 4F6-9 3F3-19

21. 4F6-6

22. TATA Transcription Start Site G9.17

23. 4F6-19

24. The Gene Network of E.Coli: (bacterium, single cell) (U. Alon)

25. p53 CDE / E2F CHR ELK1 NFY CDK4 CDK6 CDKN1A (p21) E2F RB NFY CDKN2A (p16) Mitogens MAPK ELK 1 1.M.Milyevsky,Y.Tabach, ….T.Pilpel,V. Rotter – in vitro 2. C. Rosty,M. Sheffer, D.Tsafrir….in vivo, cervical cancer CDK2 Cervical cancer induced by HPV: two viral oncoproteins (E6,E7) are inserted in DNA and expressed. They inactivate p53 and Rb and induce proliferation Inactivation by E7 binding Degradation Induced by E6

26. 4F6-21

27. 3-16 Genetic code 3 READING FRAMES

29. beta-globin(human)sequences VHLTP(EEKSAVTALW){GK} VN(VDEVGGEALGRLLVV)Y (PWTQRF)F(ESF)GDLST (PDAVM){G}N (PKVKAHGKKVLGAFSDGL) {AH}(LDNLKGTFATLSELHCD) {KL}HVD{P} (ENFRLLGNVLVCVLAHHFGKE)FT (PPVQAAYQKVVAGVANALA) {HK}YH

30. 3FtRNA

31. 6-8,9 tRNA 6-8,9

32. 4F6-653FtRNA

33. 4F6-58 4F6-60

34. polyribosome 3F6-28,29

36. Central Dogma Transcription mRNA Cells express different subset of the genes In different tissues and under different conditions Gene (DNA) Translation Protein