1. C H. 6 W ARM -U P 1.What are the 2 main types of cells? Which Domains do they consist of? 2.List 3 ways that eukaryotes differ from prokaryotes.

2. C H. 6 W ARM -U P 1.How is the size of a cell related to its function? 2.Name 5 organelles or cell structures and their function.

3. W ARM -U P A CTIVITY : Pick up handout on back counter. Complete this handout for your warm-up activity.

4. C H. 6 W ARM -U P Animal CellPlant Cell Compare and contrast Animal vs. Plant Cells

5. C H. 6 W ARM -U P What is the structure & function of: 1. Microtubules 2. Microfilaments 3. Intermediate filaments

6. C H. 6 W ARM -U P What is the function of: 1. Plasmodesmata 2. Gap junctions 3. Tight junctions 4. Desmosomes

7. C HAPTER 6 A Tour of the Cell

8. Y OU M UST K NOW Three differences between prokaryotic and eukaryotic cells. The structure and function of organelles common to plant and animal cells. The structure and function of organelles found only in plant cells or only in animal cells.

9. H OW W E S TUDY C ELLS Biologists use microscopes and the tools of biochemistry to study cells

10. Size range of cells Note that light microscopes can not magnify as well as electron microscopes

11. Light Microscopy (LM) vs. Electron Microscopy (EM)

12. 2 T YPES OF C ELLS : 1. Prokaryotes : Domain Bacteria & Archaea 2. Eukaryotes (Domain Eukarya): Protists, Fungi, Plants, Animals

13. A P ROKARYOTIC C ELL ( BACTERIA )

14. P ROKARYOTE V S. E UKARYOTE “before” “kernel” No nucleus DNA in a nucleoid Cytosol No organelles other than ribosomes Small size Primitive i.e. Bacteria & Archaea “true” “kernel” Has nucleus and nuclear envelope Cytosol Membrane-bound organelles with specialized structure/function Much larger in size More complex i.e. plant/animal cell

15. C ELL S IZE AND S CALE http://learn.genetics.utah.edu/content/begin/cells/scale/ Scale of the Universe: http://www.onemorelevel.com/game/scale_of_the_unive rse_2012

16. surface area to volume ratio Cells must be small to maintain a large surface area to volume ratio Large S.A. allows  rates of chemical exchange between cell and environment

17. A NIMAL S URFACE A REA E XAMPLE (A NIMAL ): Small Intestine: highly folded surface to increase absorption of nutrients Villi Villi: finger-like projections on SI wall Microvilli Microvilli: projections on each cell

18. F OLDS  V ILLI  M ICROVILLI

19. P LANT S URFACE A REA E XAMPLE (P LANT ): Root hairs Root hairs : extensions of root epidermal cells; increase surface area for absorbing water and minerals

20. N UCLEUS Function: control center of cell Contains DNA Surrounded by double membrane ( nuclear envelope ) Continuous with the rough ER Nuclear pores : control what enters/leaves nucleus Chromatin : complex of DNA + proteins; makes up chromosomes Nucleolus : region where ribosomal subunits are formed

21. N UCLEUS Contains DNA Function: control center of cell Surrounded by double membrane ( nuclear envelope ) Continuous with the rough ER Nuclear pores : control what enters/leaves nucleus Chromatin : complex of DNA + proteins; makes up chromosomes Nucleolus : region where ribosomal subunits are formed

22. R IBOSOMES Function: protein synthesis Composed of rRNA + protein Large subunit + small subunit Types: 1. Free ribosomes : float in cytosol, produce proteins used within cell 2. Bound ribosomes : attached to ER, make proteins for export from cell

23. E NDOMEMBRANE S YSTEM : Regulates protein traffic & performs metabolic functions

24. E NDOPLASMIC R ETICULUM (ER) Network of membranes and sacs Types: 1. Rough ER : ribosomes on surface Function: package proteins for secretion, send transport vesicles to Golgi, make replacement membrane 2. Smooth ER : no ribosomes on surface Function: synthesize lipids, metabolize carbs, detox drugs & poisons, store Ca 2+

25. E NDOPLASMIC R ETICULUM (ER)

26. G OLGI A PPARATUS Function: synthesis & packaging of materials (small molecules) for transport (in vesicles); produce lysosomes Series of flattened membrane sacs (cisternae) Cis face : receives vesicles Trans face : ships vesicles

27. L YSOSOMES Function: intracellular digestion; recycle cell’s materials; programmed cell death (apoptosis) Contains hydrolytic enzymes

28. V ACUOLES Function: storage of materials (food, water, minerals, pigments, poisons) Membrane-bound vesicles Eg. food vacuoles, contractile vacuoles Plants: large central vacuole -- stores water, ions

32. Parts of plant & animal cell p 108-109

34. M ITOCHONDRIA Function: site of cellular respiration Double membrane: outer and inner membrane Cristae : folds of inner membrane; contains enzymes for ATP production; increased surface area to  ATP made Matrix : fluid-filled inner compartment

35. C HLOROPLASTS Function: site of photosynthesis Double membrane Thylakoid disks in stacks (grana); stroma (fluid) Contains chlorophylls (pigments) for capturing sunlight energy

36. I LLUSTRATIVE E XAMPLES : Variations within molecules provide a wider range of functions: Chlorophylls

37. E NDOSYMBIONT THEORY Mitochondria & chloroplasts share similar origin Prokaryotic cells engulfed by ancestors of eukaryotic cells Evidence: Double-membrane structure Have own ribosomes & DNA Reproduce independently within cell

38. P EROXISOMES Functions: break down fatty acids; detox alcohol Involves production of hydrogen peroxide (H 2 O 2 )

39. C YTOSKELETON : NETWORK OF PROTEIN FIBERS Function: support, motility, regulate biochemical activities

40. MicrotubulesMicrofilaments Intermediate Filaments Protein = tubulin Largest fibers Shape/support cell Track for organelle movement Forms spindle for mitosis/meiosis Component of cilia/flagella Protein = actin Smallest fibers Support cell on smaller scale Cell movement Eg. ameboid movement, cytoplasmic streaming, muscle cell contraction Intermediate size Permanent fixtures Maintain shape of cell Fix position of organelles 3 T YPES OF C YTOSKELETON F IBERS :

41. MicrotubulesMicrofilaments Intermediate Filaments 3 T YPES OF C YTOSKELETON F IBERS :

43. Centrosomes : region from which microtubules grow Also called microtubule organizing center Animal cells contain centrioles

44. C ILIA & F LAGELLA Flagella: long and few; propel through water Cilia: short and numerous; locomotion or move fluids Have “9+2 pattern” of microtubules

45. E XTRACELLULAR M ATRIX (ECM) Outside plasma membrane Composed of glycoproteins (ex. collagen) Function: Strengthens tissues and transmits external signals to cell

46. I NTERCELLULAR J UNCTIONS (A NIMAL CELLS ) Tight junctions : 2 cells are fused to form watertight seal Desmosomes : “rivets” that fasten cells into strong sheets Gap junctions : channels through which ions, sugar, small molecules can pass

47. P LANT C ELLS Cell wall: protect plant, maintain shape Composed of cellulose Plasmodesmata : channels between cells to allow passage of molecules

48. Plant Cells OnlyAnimals Cells Only Central vacuolesLysosomes ChloroplastsCentrioles Cell wall of celluloseFlagella, cilia Plasmodesmata Desmosomes, tight and gap junctions Extracellular matrix (ECM)

49. H ARVARD CELL VIDEO http://multimedia.mcb.harvard.edu/anim_innerlife.html