1. INVESTIGATING S100A4 AS THERAPEUTIC TARGET VIS-À-VIS EMPLOYABILITY OF AYURVEDIC CONCEPTS FOR ADVANCED RESEARCH AND CLINICAL RELEVANCE Vivek Sharma Scientist In-charge Division of Cancer Research Department of Biotechnology Chandigarh Group of Colleges, Mohali & Founder, Anvaya Biosciences (a start-up company) Ph. No. +91-8054 66 7211; E-mail: biotechno16@gmail.com

2. S100A4 AT A GLANCE !

3. FOCUS OF RESEARCH Structure of MAP Signalling pathways Molecular target Inhibition via mutagenesis

4. IMPORTANT RESULTS S100A4 under normal conditions does not affect the cell polarity. S100A4 triggers JNK pathway in metastatic conditions. Analyzing S100A4 as a therapeutic target.

5. INTERACTION BETWEEN FENUGREEKINE AND 3C1V FENUGREEKINE Fig. (1a) Distances between active site residues of 3C1V and Fenugreekine in Angstrom (Å) Fig. (1b) Fitting of Fenugreekine in the active site cavity of 3C1V.

6. INTERACTION BETWEEN GERANIOL AND 3C1V GERANIOL Fig. 2: Fitting of Geraniol in the active site cavity of 3C1V.

7. SIMULATION STUDIES Fig. 3: Simulation studies and comparison of potential energies and temperature parameters between (a) 3C1V, (b) 3C1V-Fenugreekine and (c) 3C1V-Geraniol complex. a b a bc c

8. QUESTION IN FOCUS! In the current day clinical practice, what treatment strategy is adopted to treat a patient with metastasis? Best answer one can get is combinatorial therapies. Looking at new targets New mutations Finding out new molecules

9. THE TRANSLATIONAL SIGNIFICANCE! Yes they are. But, how much comprehensively these therapies are applicable? What is the success rate? Are these therapies treating the disease or just managing it? Are the fields of genomics, proteomics and metabolomics really helping us in finding out the therapies for diseases? Are the processes of pre-clinical trials, clinical trials (Phase I, II, III) and the pharmacovigilance robust enough?

10. SYSTEMS APPROACH MAJOR ELEMENTS AND REQUIREMENTS OF SYSTEMS BIOLOGY Molina F et al. (2010)

11. AYURVEDA Ayurveda is Upveda of ‘Athraveda’ and its practice can be traced back to 3000 BCE. Is the oldest system of medicine known in the world with reported evidences by the sages and saints of ancient India. It deals with the holistic understanding of the disease, followed by holistic treatment.

12. B ASIC P RINCIPLES OF A YURVEDA Principle of Tridosha – Vata, Pitta, Kapha Principle of Panchmahabhutas – Akash (Space), Vayu (Air), Jal (Water), Agni (Fire), Prithvi (Earth) Principle of Saptdhatu – Rasa (Plasma), Rakta (blood cells), Mamsa (muscle tissue), Meda (fatty tissue), Asthi (bone tissue), Majja (bone marrow and nervous tissue), Shukra (reproductive tissues) Principle of Srotvijanana – which is about Macrocosm – microcosm continuum and it correlates with Quantum Biology concept. Discipline of Bhaisjya Kalpana – field of Drug delivery in Ayurveda

14. INTEGRATING THE KNOWLEDGE! Can we integrate the knowledge of Ayurveda with the tools of genomics and proteomics to build a platform which can be used for development of truly integrative and personalized medicine? Can we bring in the original principles of Ayurveda in modern day research models?

15. R EPORTS

16. HOW CAN WE DO IT?

17. I NTEGRATING THE SCIENCE OF A YURVEDA WITH MODERN TECHNOLOGIES www.anvayabio.com E-mail - anvayabios@gmail.com

18. OUR FOCUS To create a amalgamated platform for bringing together the molecular diagnostics, Ayurveda diagnostics and treatment strategies from both fields with personalized approach. To create a library of plant based compounds and there derivatives having potential therapeutic effect in cancer (for example). To create awareness among Ayurveda fraternity for importance of molecular tools and techniques and how these tools can be helpful in validating the ancient knowledge for its global acceptance.

19. ACKNOWLEDGEMENTS Prof. (Dr.) Upendra K. Jain, Director – Principal, Chandigarh College of Pharmacy, CGC, Mohali. Mrs. Palki Sahib Kaur, HOD – Dept. of Biotechnology, CGC, Mohali. Collaborators: Mr. Rakesh Kr. Pandit, Assistant Professor, Bioinformatics, Dept. of Biotechnology, CGC. Mrs. Piyusha Sharma, Anvaya Biosciences Mr. Aniket Sinha, Anvaya Biosciences Students: Navpreet Kaur Shikha Pooja Thakur Manpreet Kaur

20. THANK YOU QUESTIONS ARE INVITED!