2. BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals Video camera Water pool Hidden Platform Animal (rat) B V

3. BVisionBVision Data Tracking System Analyzing Tools Acquisition Management Visualization Analysis Configuration parameters B V Automatic creation of the video archive

4. B V Program correction of the video path in the framegrabber. Minimizing load of the processor. Fast signal proceeding (up to 25 frames/s Displaying zones boundaries Live Video

5. Data structure and storage Flexible database management system. Tracks and Experiments Stored in the Database B V

6. Arena and zones definition Automatical detection of borders of the pool and other analyzed zones (for example, platform) Result of Zones Detection Restoration of the Platform Position B V

7. Object Detection Algorithm Формальный нейрон Framegrabber Input video signal Program correction of the video path Differentiation of all the found objects by size Corrected video signal Detected animal 1 Differentiation of the residual objects according to physical reasons 2 B V

8. Tracking process B V

9. Parameters B V escape latency, accounting for time of active swimming only (with speed lower than selected threshold), trajectory length, speed, index of “pasive” swimming” (to characte- rize decrease of animals’ mobility), time in zone of interest (ZOI), % of tracks failed to find the platform, straightness index (deviation from the direct path to platform), cumulative and reverse cumulative distances to platform, angle characteristics of movement relative platform, Whishaw “alley” index, etc.

10. Analysis of a Single Track B V

11. Zones Definitions for Analysis B V

12. Main Analysis Window B V

13. Results Visualization B V