1. Circadian rhythm March 14 1088 tongli zhang

3. Circadian rhythm

4. Circadian rhythms in different organisms

5. Control network in mammalian

6. Circadian rhythms in Cyanobacyteria

7. cyanobacteria

9. Their interaction KaiA, KaiB and KaiC interact with each other, forming large complexes in vivo. KaiC is the core of the complex. The complex is mainly at night.

10. kaiC

11. KaiA kaiA enhances KaiC phosphorylation KaiA has two domains- N-terminal is a “pseudo-receive” domain N-terminal domain of KaiA does not promote KaiC autophosphorylation KaiA from anabaena even does not have a N-terminal domain However, mutation of N-terminal domain of KaiA lengthen the circadian period

12. KaiA 2 KaiA C-terminal binds KaiC and promotes its phosphorylation One KaiA is enough to enhance the phosphotylation of one KaiC hexamer

13. KaiA 3 KaiA exists as a domain-swapped homodimer KaiA's pseudo-receiver domain The C-terminal domain of KaiA, C-KaiA, is all a- helical and adopts a novel dimeric structure.

14. KaiA 4 open and close kaiA dimer N of one and C of another interact This is closed When the interaction is broken- KaiA dimer is open Closed is supposed to be the active form

15. kaiC binding with KaiA

16. kaiB Positively charged residues R23 and K75

17. kaiC binds with KaiB The potentials (positive, blue; negative, red)

18. A rotary clock

19. Happy everyone? What about 1. entrainment? 2. temperature compensation? 3. fitness? 4. cancer development?

20. Way to solutions.