1. LAB ON A CHIP GROUP R Josh Epperson, Brooke Ott, Luisa Parish, and Ben Weisman

2. BRAINSTORMING ● Generic Chip Design Issues ● Backflow, Air Bubbles ● Shape of Staging Wells ● Circlular vs. Elliptical ● Shape of Channels Straight vs. Curved ● Number and Location of Check Valves ● 2 Check Valve System

3. FEATURES OF FIRST CHIP ● 4 Staging Wells o 2 circular o 2 elliptical ● Number of Check Valves o 2 versus 1 ● Straight Channels

4. RESULTS AND OBSERVATIONS OF FIRST CHIP DESIGN ● Circular staging more effective and simplistic ● Multiple check valves ineffective ● No impact on backflow Standard deviation of 8.60

5. FIRST CHIP CALIBRATION CURVES

6. FEATURES OF SECOND CHIP Circular Staging Wells Channels meet before going to Detection Well

7. RESULTS OF SECOND CHIP More consistent results Easier injection into chip Less air bubbles in the detection well Standard deviation of 4.19

8. SECOND CHIP CALIBRATION CURVES

9. CONCLUSION Second chip preferred More accurate and simple Equation for ppm used was 0.153x 2 - 3.85x+36.976. Average deviation was 10.67% for all samples.