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A b c d 30% AcN 30% Organic (143 Features) FT 200 500 1000 #Features25 18 43 43 CV0.2 0.3 0.3 0.5 R 2 0.99 0.98 0.96 1.0 45% Organic (237 Features) FT.

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Presentation on theme: "A b c d 30% AcN 30% Organic (143 Features) FT 200 500 1000 #Features25 18 43 43 CV0.2 0.3 0.3 0.5 R 2 0.99 0.98 0.96 1.0 45% Organic (237 Features) FT."— Presentation transcript:

1 a b c d 30% AcN 30% Organic (143 Features) FT 200 500 1000 #Features25 18 43 43 CV0.2 0.3 0.3 0.5 R 2 0.99 0.98 0.96 1.0 45% Organic (237 Features) FT 200 500 1000 #Features52 56 62 67 CV0.2 0.2 0.2 0.3 R 2 0.98 0.99 0.98 0.99 80% Organic (258 Features) FT 200 500 1000 #Features49 73 68 63 CV0.4 0.2 0.3 0.3 R 2 0.99 0.99 0.97 0.91 Method Supplemental Figure 1.

2 Pooled Serum 1) IgY-R7 Depletion Column 2) SPE w/Peptide Mactrotraps (C18/ SCX) 3) C4-RP HPLC (LC-MALDI-Spotter) 4) LC-MALDI-TOF-MS (isolate key fractions) 5) 1D-PAGE (In-gel tryptic digestion) 6) nLC-MS2 Validation by Western Blot Feature Increased in Kras vs. Control 6,560 6,620 m/z 2,000 1,500 500 0 Intensity C. Ras 6,568, m/z 100 50 0 Rel. Intensity 300 1,200 m/z MS2 Sequence Protein ID a. b. c. Supplemental Figure 2.

3 Extracellular Membrane Cytoplasm Nuclear Significant Hit Receptor Ligand Transcripton Factor Generic Protein Binding Protein Transporter Receptor Generic Enzyme Protease Kinase Legend Supplemental Figure 3.


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