Presentation is loading. Please wait.

Presentation is loading. Please wait.

Advancing X-ray Crystallography via Protein Structure Prediction Acknowledgements: Dr. Jingfen Zhang Tran Nguyen Dr. Rajkumar Bondugula Dr. Dong Xu Dr.

Published byBruce Pierce Modified over 9 years ago

Similar presentations

Presentation on theme: "Advancing X-ray Crystallography via Protein Structure Prediction Acknowledgements: Dr. Jingfen Zhang Tran Nguyen Dr. Rajkumar Bondugula Dr. Dong Xu Dr."— Presentation transcript:

1 Advancing X-ray Crystallography via Protein Structure Prediction Acknowledgements: Dr. Jingfen Zhang Tran Nguyen Dr. Rajkumar Bondugula Dr. Dong Xu Dr. Jack Tanner NIH grant 2-T15-LM07089-16 Christopher Bottoms University of Missouri-Columbia December 1, 2007

2 protein crystal X-ray beam detector Intensities Phases

3 Sources of Phase Information Additional Experimental Steps –Modify crystal –Modify protein –Collect additional data Similar Protein Structures –Proteins with 30-100% seq ID –Predicted protein structures

4 ALA Initial map calculated from X-ray data and poly-alanine predicted structure. Final map calculated from X-ray data and final structure. TYR 1oot

5 Map calculated from X-ray data and all-atom predicted structure Predicted (all atom) 1k33

6 Final Final map calculated from X-ray data and final structure. Final 1k33

7 Final Predicted (all atom)

8 Future Directions Christopher@moleculesoflife.com Improving predictions for larger proteins Utilizing general selection criteria

Download ppt "Advancing X-ray Crystallography via Protein Structure Prediction Acknowledgements: Dr. Jingfen Zhang Tran Nguyen Dr. Rajkumar Bondugula Dr. Dong Xu Dr."

Similar presentations

11/9/99ICTAI-99, Chicago1 Protein Secondary Structure Prediction Using Data Mining Tool C5 Meiliu Lu †, Du Zhang †, Hongjun Xu †, Ken Tse-yau Lau ‡, and.

11/9/99ICTAI-99, Chicago1 Protein Secondary Structure Prediction Using Data Mining Tool C5 Meiliu Lu †, Du Zhang †, Hongjun Xu †, Ken Tse-yau Lau ‡, and.
Lecture 21 (12/11/2006) X-Ray Diffraction (XRD) Theory and Analytical Technique.

Lecture 21 (12/11/2006) X-Ray Diffraction (XRD) Theory and Analytical Technique.
Protein x-ray crystallography

Protein x-ray crystallography
Introduction to protein x-ray crystallography. Electromagnetic waves E- electromagnetic field strength A- amplitude  - angular velocity - frequency.

Introduction to protein x-ray crystallography. Electromagnetic waves E- electromagnetic field strength A- amplitude  - angular velocity - frequency.
Determination of Protein Structure. Methods for Determining Structures X-ray crystallography – uses an X-ray diffraction pattern and electron density.

Determination of Protein Structure. Methods for Determining Structures X-ray crystallography – uses an X-ray diffraction pattern and electron density.
X-Ray Crystallography

X-Ray Crystallography
CHE-20031 (Structural Inorganic Chemistry) X-ray Diffraction & Crystallography lecture 3 Dr Rob Jackson LJ1.16, 01782 733042

CHE (Structural Inorganic Chemistry) X-ray Diffraction & Crystallography lecture 3 Dr Rob Jackson LJ1.16,
Structural Genomics – an example of transdisciplinary research at Stanford Goal of structural and functional genomics is to determine and analyze all possible.

Structural Genomics – an example of transdisciplinary research at Stanford Goal of structural and functional genomics is to determine and analyze all possible.
Protein structure determination. Tertiary protein structure: protein folding Three main approaches: [1] experimental determination (X-ray crystallography,

Protein structure determination. Tertiary protein structure: protein folding Three main approaches: [1] experimental determination (X-ray crystallography,
(X-Ray Crystallography) X-RAY DIFFRACTION. I. X-Ray Diffraction  Uses X-Rays to identify the arrangement of atoms, molecules, or ions within a crystalline.

(X-Ray Crystallography) X-RAY DIFFRACTION. I. X-Ray Diffraction  Uses X-Rays to identify the arrangement of atoms, molecules, or ions within a crystalline.
MCP 1 L. Zhang and M. T. Lusk Colorado School of Mines T.J. Bartel and E.A. Holm Sandia National Laboratories March 18, 2008 Anisotropic EBSD Nickel data.

MCP 1 L. Zhang and M. T. Lusk Colorado School of Mines T.J. Bartel and E.A. Holm Sandia National Laboratories March 18, 2008 Anisotropic EBSD Nickel data.
Hanging Drop Sitting Drop Microdialysis Crystallization Screening.

Hanging Drop Sitting Drop Microdialysis Crystallization Screening.
A Pyroelectric Crystal Particle Accelerator

A Pyroelectric Crystal Particle Accelerator
Mark J. van der Woerd1, Donald Estep2, Simon Tavener2, F

Mark J. van der Woerd1, Donald Estep2, Simon Tavener2, F
Phase Difference = 0.00. Phase Difference = 0.05.

Phase Difference = Phase Difference = 0.05.
A Desktop Particle Accelerator Employing a Pyroelectric Crystal Jon Kalodimos, Kansas State University Dr. Rand Watson, Texas A&M Cyclotron Institute.

A Desktop Particle Accelerator Employing a Pyroelectric Crystal Jon Kalodimos, Kansas State University Dr. Rand Watson, Texas A&M Cyclotron Institute.
X-ray Diffraction Outline Crystals and Bragg Diffraction

X-ray Diffraction Outline Crystals and Bragg Diffraction
Protein Structure Determination Part 2 -- X-ray Crystallography.

Protein Structure Determination Part 2 -- X-ray Crystallography.
23 May 2002 - 2 June 2002. 24 May 2002 From genes to drugs via crystallography 19 May 1996 Experimental and computational approaches to structure based.

23 May June May 2002 From genes to drugs via crystallography 19 May 1996 Experimental and computational approaches to structure based.
 Four levels of protein structure  Linear  Sub-Structure  3D Structure  Complex Structure.

 Four levels of protein structure  Linear  Sub-Structure  3D Structure  Complex Structure.

Similar presentations

Ads by Google