1. Workshop practical Helsinki Workshop September 2006

2. Initial setup -Log in and checkout the biomart perl code: cvs -d :pserver:cvsuser@cvs.sanger.ac.uk:/cvsroot/CVSmaster login cvs -d :pserver:cvsuser@cvs.sanger.ac.uk:/cvsroot/CVSmaster co biomart-web -Add the biomart-web/lib/BioMart directory to your PERL5LIB. -Install the Exception::Class and XML::Simple modules from CPAN. perl -MCPAN -e shell install XML::Simple Exception::Class -Run the example API script to check everything is installed correctly: perl biomart-web/scripts/new_0_5_exampleSimple.pl -Run the webservices example: perl biomart-web/script/webExample.pl Query.xml

3. API exercises Write API scripts to perform the following tasks: (1)Recover the gene and transcript stable IDs along with the chromosome name for human Ensembl genes on chromosome 1. (2)Further narrow down (1) to only genes between positions 1p36.33 and 1q12. (3)Recover the gene and transcript stable IDs and HGNC symbol identifier for human Ensembl genes that match a HGNC symbol of FGFR1, IL2 or DERL3. (4)For the same genes as in (3) recover the peptide sequence with Ensembl gene and transcript stable Ids as header attributes in FASTA output format. (5)Recover the human gene stable ID, human transcript stable ID, human chromosome, mouse gene stable ID, mouse transcript stable ID and mouse chromosome for human, chromosome 1 Ensembl genes with a mouse ortholog on mouse chromosome 2.

4. Martservice exercises Write Query XMLs and run with webExample.pl to perform the same tasks: (1)Recover the gene and transcript stable IDs along with the chromosome name for human Ensembl genes on chromosome 1. (2)Further narrow down (1) to only genes between positions 1p36.33 and 1q12. (3)Recover the gene and transcript stable IDs and HGNC symbol identifier for human Ensembl genes that match a HGNC symbol of FGFR1, IL2 or DERL3. (4)For the same genes as in (3) recover the peptide sequence with Ensembl gene and transcript stable Ids as header attributes in FASTA output format. (5)Recover the human gene stable ID, human transcript stable ID, human chromosome, mouse gene stable ID, mouse transcript stable ID and mouse chromosome for human, chromosome 1 Ensembl genes with a mouse ortholog on mouse chromosome 2.