1. circulation KIDNEYS ureters bladder Glomerulus (plasma filtration)‏ Tubule (reabsorption)‏ urethra URINARY-PROTEOME to the ureter 30% circulation 70% urogenital tract Figure 1 primitive urine = reabsorption NEPHRON final urine

2. Figure 2 Ureteropelvic junction (UPJ) obstruction Spontaneous resolution Relief surgery (pyeloplasty)‏ ? Intermediate obstruction Spontaneous resolution pyeloplasty birth1 to 2 years scintigraphies A B –1 –0.5 0 0.5 1 Membership 9 months spontaneous resolution surgery Clinical evolution 15 months Prediction based on urinary proteomics

3. Figure 3 LARGE DYNAMIC RANGE Ligand peptide library (millions of different ligands)‏ Flow-Through: Highly abundant proteins REDUCED DYNAMIC RANGE +