1. GMO’s What do you know about GMO’s?
What it stands for ?
How are they made?
What are they used for?
Examples of GMO’s
Companies that use GMO’s
Do you think we should use GMO’s? Why?
Write your answers/comments in the first block of your Yellow Thinking Map.

2. Are genetically modified organisms safe?
Articles
Read your assigned article:
“Taco Bell Will Replace Taco Shells from Restaurants” ABC News
“Is Genetically Modified Salmon Safe?” Science Daily
Highlight your article with information that will help answer the question:
Are genetically modified organisms safe?
Discuss the article within your group. Get one highlighted piece of information from the other article. Record two things you learned in the second chart on your yellow thinking map.

3. Biotechnology Test with DNA and Protein Synthesis
DNA Technology/3.0
Biotechnology
Test with DNA and Protein Synthesis

4. Reminders about DNA:
All cells have DNA.
DNA looks the same in all organisms but codes for different things.
The DNA is just ATCG’s. It cannot do anything outside of a cell. It needs enzymes and ribosomes to make protein.
One cell could use another cell’s DNA if it was inserted into the organism.

5. Restriction Enzyme: Enzyme that can cut the strong bonds of DNA.
Recombinant DNA
Genes from different sources are combined.
Ex. Frost resistance gene from potatoes put into strawberry DNA.
Terms to know:
Vector
The vector carries target DNA into a living cell.
Example: Plasmid-Circular piece of bacterial DNA
Donor Gene
The target gene that scientists want to use from an organism.
Restriction Enzyme: Enzyme that can cut the strong bonds of DNA.

6. How do scientists recombine the DNA?
Identify and isolate the target gene from the donor organism using restriction enzymes.
Cut the vector DNA open using the same restriction enzyme.
Then you put the target gene inside the vector (plasmid). They stick together like tape.
**Must be returned to a living cells**
When an organism receives this new recombined DNA it is called TRANSGENIC.

7. Restriction enzymes cut the DNA in a specific spot
Restriction enzymes cut the DNA in a specific spot. Sticky ends are open pieces of DNA that attach and seal to the new gene with ligase. Sticky Ends Plasmid

8. Transgenic Organisms
Living things that contain recombined DNA (DNA from another organism).
After the gene has been put into the new DNA it must be placed in a living cell.
These organisms can be used in:
Pharmaceuticals
Medical
Agriculture
Environmental Solutions

9. Creating Human Insulin
Got Silk? Video
Watch the video. Write down two interesting pieces of information in your notes.
Creating Human Insulin

10. Transgenic bacteria producing HUMAN insulin.

11. Diabetes Articles-one paper to turn in!
Read the diabetes articles at your table and work together to answer the questions. (15 min)
1. What is insulin?
2. Why is insulin necessary in your body?
3. When does type 1 diabetes usually appear?
4. Because of sugar in the urine, diabetes was originally thought to be a disease of which organ?
5. What are some problems with using beef and pork insulin?
6. What is the difference between type 1 and type 2 diabetes?
7. What are some long term complications of diabetes?
8. Think! What would be the best solution to this problem for people with diabetes?

13. Pope Francis gave his personal blessing to Golden Rice (GR)
Pope Francis gave his personal blessing to Golden Rice (GR). Why is this significant? Vitamin A deficiency (VAD) is responsible for 500,000 cases of irreversible blindness and up to 2 million deaths each year. Particularly susceptible are pregnant women and children. Across the globe, an estimated 19 million pregnant women and 190 million children suffer from the condition. The good news, however, is that dietary supplementation of vitamin A can eliminate VAD. One way that holds particular promise is the administration via Golden Rice, which had been engineered to produce large amounts of vitamin A.

14. Fill in two more numbers on the second block of your thinking map with information from the notes, video clip and insulin diagram.
Make sure your name is on your sheet and return the yellow paper to Mrs. Handest.

15. Diagrams

16. DNA Fingerprinting RFLP-Restriction Fragment Length Polymorphism
Everyone has these sections of DNA but the restriction enzymes cut them into different sized pieces.
The way your DNA cuts is specific to you.
How do we see the pieces?
Gel Electrophoresis

17. Gel Electrophoresis
Separates DNA according to the size of the pieces (fragments)
DNA is put into a gelatin like substance and an electric charge is applied.
Since DNA is negatively charged which direction will it move?
Large pieces get “stuck” in the gel and smaller pieces can move further.

18. Uses for Gel Electrophoresis
DNA fingerprints for:
Forensics
Paternity testing
Sequencing DNA

19. practice

20. PCR Polymerase Chain Reaction
Used to make thousands of copies of one tiny piece of DNA.
Uses-
Forensics
To isolate (find) a gene

21. Cloning
Read the cloning article and answer the following questions in a blank space on your notes:
How many “mothers” did Dolly the cloned sheep have?
Use pictures to explain the process used to create Dolly.
What are some uses of cloned organisms?
(15 minutes)

22. Cloning Organisms
Myths: 1. The cloned organism will be identical in every way to the original Clones cannot reproduce Clones look the same but some genes are different.
Truth: Cloning creates a new organism that has genes that are identical to the original organism.
Steps
Take a full set of DNA from one cell of an organism.
Put the DNA into a stem cell
Insert cell into an adult organism (if an animal) and allow the cell to develop into a full organism.

23. Why Cloning? If it ain’t broke don’t fix it.
Make many copies of cells/ organs in the lab for use in medicine.

24. Plant Cloning very simple and has been done for centuries

25. Human Genome Project
Project to identify all genes in human genome, determine the sequence of base pairs that make up human DNA
Completed in 2003
Accomplished using gene sequencing by gel electrophoresis(determining the exact order of bases in DNA)

26. HGP continued Eventually HGP info can help us with Gene Therapy
Correcting defective genes responsible for disease development through replacement with other genes
Not yet widespread and successful with humans.

27. U4HW3 Technology How does it work?/What is it?
How is it useful to humans? Examples.
Recombinant DNA
Enzymes that cut DNA
Gel Electrophoresis and recombinant DNA
Separates DNA fragments based on size.
Cloning
Exact copies of cells or organisms.
Human Genome Project
Gene Therapy

28. Why is this important?
Biotechnology-use of living organisms to benefit society.
Genetic Engineering-manipulating genes of organisms to benefit society.
Applications? Forensics, Agriculture, Medicine, Pharmaceuticals, Others?….