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Published byAlexander O’Brien’ Modified over 9 years ago
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VISCOSITY Transport property defined as the retarding effect of slower layers of a fluid on an adjacent faster layer.
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VISCOSITY Viscosity is a transport property that is observed when there is a momentum or velocity gradient. It is defined as the retarding effect of a slower layer of a fluid on an adjacent faster layer. Flux = J x α -dv/dx where the proportionality constant = η = viscosity coefficient or constant ( g/(cm-s)
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Experiment 1. Measure viscosity by measuring time (t) it takes a fluid to fall between two marks of viscometer. ηρ = Bt where ρ = fluid density and B = viscometer constant. 2. Determine B using water 3. Determine η of BSA as a function of its concentration
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Experiment (2) 4. Determine effective spherical radius of BSA protein. 5. Repeat steps 3 and 4 for denatured BSA.
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Two-Day Expt Day 1: Native BSA: make stock solution and its dilutions; measure η = f(c). Denatured BSA: make stock solution Day 2: d-BSA: make dilutions and measure η = f(c).
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