Presentation is loading. Please wait.

Presentation is loading. Please wait.

1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending 1 The Microcapillary Protein.

Published byClement Marshall Modified over 9 years ago

Similar presentations

Presentation on theme: "1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending 1 The Microcapillary Protein."— Presentation transcript:

1 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending 1 The Microcapillary Protein Crystallization System MPCS TM This work supported in part by NIGMS NCRR co-sponsored PSI-2 Grant U54 GM074961 Cory Gerdts Enabling Technologies Workshop 3/4/09

2 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending MPCS Competitive Advantages: Low Volume (5-10 nL Protein / experiment) Diffraction-Ready TM Crystals – translation / scale up not required Crystals easily extracted from Peel-Apart TM CrystalCards No Dead Volume – Every nL Gets Used In situ diffraction ~22  L performs a gradient of 96 conditions 2

3 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending Zheng B.; Roach L. S.; R. F. Ismagilov J. Am. Chem. Soc. 2003, 125: 11170-11171. Plug-Based Protein Crystallization Zheng, B.; Ismagilov, R.F. Angew. Chem. 2005, 117: 2576-2579. H. Song, J. D. Tice, R. F. Ismagilov Angew. Chem.-Int. Edit. 2003, 42, 768.

4 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending CrystalCard™ 20 nl Plugs 200  m microcapillary How Does it Work? 4

5 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending CrystalCard™ 20 nl Plugs 200  m microcapillary 5 How Does it Work?

6 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending

7 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending Efficient Optimization of Crystal Growth by Fine Gradient Screening pH 6pH 7 pH 8 Optimize crystal growth using fine control over chemical gradients for: Protein, Buffers (pH), Salts, Ligands, Additives, Precipitants, etc. 20 nl Plugs 7

8 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending A Smooth Chemical Gradient with the MPCS Single Crystal Precipitate Microcrystals Single Crystals Crystal Clusters Gerdts et al. Acta Cryst. (2008). D64, 1116–1122. 8

9 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending Bups 27 SSGCID and the MPCS Methionine-R-sulfoxide reductase of Burkholderia pseudomallei Bups.00033.a 1.7 Å PDB ID: 3CXK 4-24-08 Bups.00014.b.A1 2.9 Å PDB ID: 3EOO Gerdts et al. Acta Cryst. (2008). D64, 1116–1122 Bups 69 Bups 35 Bups 75 Bups 33 + Met Bups 14 Bups 33 9

10 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending

11 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending Spacer Gas Bubble Spacer Protein Li, L., et al. (2006). PNAS. 103, 19243. Pre-formed cartridges of crystallization reagents are combined with chemical gradients for efficient screening. ~22  L performs a gradient of 96 conditions (up to 3500 individual experiments) Hybrid Method: Sparse Matrix + Fine Chemical Gradient Screening 11

12 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending No Dead Volume – Every nL is Used The Plug Maker aspirates protein into one of the tubes connected to a syringe that is back-filled with the carrier fluid (cf). Every nL of protein gets displaced into the CrystalCard. cf Syringe filled with carrier fluid 12

13 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending detector X-Rays MPCS™ CrystalCard™ (Side view) 30° In situ X-ray Diffraction Schematic goniometer head Beam Line Requirements: In-Line Optics for Crystal Aligment Stable and Accurate Motion Control Small Intense Beam Novel High Resolution Detectors 13

14 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending detector goniometer head X-Rays MPCS™ CrystalCard™ (Side view) 30° In situ X-ray Diffraction Schematic 100  m 1 mm Beam Line Requirements: In-Line Optics for Crystal Aligment Stable and Accurate Motion Control Small Intense Beam Novel High Resolution Detectors 14

15 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending In situ X-ray Diffraction Screening and Data Collection Crystals grown in the CrystalCard were subjected - in situ – to X- rays. Data collection at the NE-CAT beamline 24ID-C at APS Data merged from 3 crystals to generate a 1.9 Å resolution electron density map In situ Screening performed at GM/CA 23B CrystalCard goniometer holder being developed Gerdts et al. Acta Cryst. (2008). D64, 1116–1122. 15

16 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending MPCS User Facility Visit MPCS User Facility ~5 miles from APS at ANL 2501 Davey Road, Woodridge, IL 60517 www.emeraldbiosystems.com Development of the NIGMS NCRR co-sponsored PSI-2 Grant U54 GM074961 16 MPCS-Beta Instrument

17 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending The Microcapillary Protein Crystallization System (MPCS™) Plug Maker™ & CrystalCards™ PSI Appreciation Discount: 30% Contact us TODAY for a Quotation: MPCS@emeraldbiosystems.com In appreciation of the ATCG3D and PSI funding, we are introducing the MPCS with a 30% discount! Discount offered for limited time only. Contact us for details.U.S. Patents #7,129,091, #6,409,832 and Patents Pending

18 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending Touch-Screen User Interface Small Footprint (~18”x15”x14”) No Dead Volume Low Volume (5-10 nL Protein / Plug) ~22  L Performs Gradient of 96 Conditions Diffraction-Ready Crystals – “Translation” not Required Automated Crystallization Screening – Sparse Matrix and Gradient Optimization Fully Integrated Experimental Setup (Hardware/Software) The Plug Maker™ 18

19 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending Automated Sampling 19

20 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending Loaded and Ready 20

21 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending MPCS Competitive Advantages: “I can’t get my crystals out” 21 MPCS: Other Microfluidic Crystallization System(s): Peel-Apart TM CrystalCards TM “Crystal hits don’t translate or scale-up to vapor diffusion” Diffraction-Ready TM crystals – translation/scale-up unnecessary “Microchips are expensive” (minimum ~$0.63 per drop) More than 3X cheaper (~$0.19 per drop)

22 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending www.emeraldbiosystems.com/MPCS 22 - Videos - e-presentation (youtube) - Slides - User Facility

23 1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending

Download ppt "1-888-780-8535 © 2008 1-888-780-8535 © 2009 www.emeraldbiosystems.com U.S. Patents #7,129,091, #6,409,832 and Patents Pending 1 The Microcapillary Protein."

Similar presentations

Crystal Quality – A Practical Guide Louis J Farrugia Jyväskylä Summer School on Charge Density August 2007.

Crystal Quality – A Practical Guide Louis J Farrugia Jyväskylä Summer School on Charge Density August 2007.
Development of Bialkali Transfer Photocathodes for Large Area Micro- Channel Plate Based Photo Detectors 1 Argonne National Laboratory, Argonne, IL 2 University.

Development of Bialkali Transfer Photocathodes for Large Area Micro- Channel Plate Based Photo Detectors 1 Argonne National Laboratory, Argonne, IL 2 University.
Microfluidic Dialysis Protein Crystallization Jiang Huang GN Biosystems, Inc. March 26, 2009.

Microfluidic Dialysis Protein Crystallization Jiang Huang GN Biosystems, Inc. March 26, 2009.
Experimental mapping of protein precipitation diagrams Morten O.A. Sommer Centre for Crystallographic Studies University Of

Experimental mapping of protein precipitation diagrams Morten O.A. Sommer Centre for Crystallographic Studies University Of
IFFAT FATIMA UOG. ELECTRON MICROSCOPE Contents History LM Vs EM Electron microscope Principle Types of EM Application & importance.

IFFAT FATIMA UOG. ELECTRON MICROSCOPE Contents History LM Vs EM Electron microscope Principle Types of EM Application & importance.
Microfluidic Hybrid Method for Simultaneous Screening and Optimization Validated with Crystallization of Membrane Proteins Liang Li, Cory Gerdts, Qiang.

Microfluidic Hybrid Method for Simultaneous Screening and Optimization Validated with Crystallization of Membrane Proteins Liang Li, Cory Gerdts, Qiang.
UWO Nanofabrication Facility and Science Studio. Facility to be hooked into Science Studio: Western Nanofabrication Facility, University of Western Ontario.

UWO Nanofabrication Facility and Science Studio. Facility to be hooked into Science Studio: Western Nanofabrication Facility, University of Western Ontario.
Tomsk Polytechnic University1 A.S. Gogolev A. P. Potylitsyn A.M. Taratin.

Tomsk Polytechnic University1 A.S. Gogolev A. P. Potylitsyn A.M. Taratin.
Slide 1Reaction Analytics Inc. ©2011 “iFlow” Flow Analytics System Reaction Analytics Inc. Makers of the iChemExploreriChemExplorer.

Slide 1Reaction Analytics Inc. ©2011 “iFlow” Flow Analytics System Reaction Analytics Inc. Makers of the iChemExploreriChemExplorer.
Structural Genomics – an example of transdisciplinary research at Stanford Goal of structural and functional genomics is to determine and analyze all possible.

Structural Genomics – an example of transdisciplinary research at Stanford Goal of structural and functional genomics is to determine and analyze all possible.
Future directions in research on biomolecular structure NSLS-II Workshop July 17,2007.

Future directions in research on biomolecular structure NSLS-II Workshop July 17,2007.
Alchemist TM II: Capacity, reproducibility and efficiency of an high-throughput crystallization screen preparation system. Craig Sterling Director of Business.

Alchemist TM II: Capacity, reproducibility and efficiency of an high-throughput crystallization screen preparation system. Craig Sterling Director of Business.
A Microfluidic System for Controlling Reaction Networks In Time Presented By Wenjia Pan.

A Microfluidic System for Controlling Reaction Networks In Time Presented By Wenjia Pan.
Hanging Drop Sitting Drop Microdialysis Crystallization Screening.

Hanging Drop Sitting Drop Microdialysis Crystallization Screening.
X-ray Crystallography Kalyan Das. Electromagnetic Spectrum 10 -1 to 10 nm 400 to 700 nm 10 -4 to 10 -1 nm 10 to 400 nm 700 to 10 4 nm X-ray radiation.

X-ray Crystallography Kalyan Das. Electromagnetic Spectrum to 10 nm 400 to 700 nm to nm 10 to 400 nm 700 to 10 4 nm X-ray radiation.
LCLS Studies of Laser Initiated Dynamics Jorgen Larsson, David Reis, Thomas Tschentscher, and Kelly Gaffney provided LUSI management with preliminary Specifications.

LCLS Studies of Laser Initiated Dynamics Jorgen Larsson, David Reis, Thomas Tschentscher, and Kelly Gaffney provided LUSI management with preliminary Specifications.
The Crystallographic Refinement of TM1389- A methyl-transferase from Thermotoga maritima Rosanne Joseph SLAC Summer Intern Joint Center for Structural.

The Crystallographic Refinement of TM1389- A methyl-transferase from Thermotoga maritima Rosanne Joseph SLAC Summer Intern Joint Center for Structural.
Output devices. There is no point in having a computer that can do wonderful things unless it can tell you the results of what it has been doing. This.

Output devices. There is no point in having a computer that can do wonderful things unless it can tell you the results of what it has been doing. This.
Laboratories of Biophysics and Nanobiotechnology

Laboratories of Biophysics and Nanobiotechnology
The Future of Structural Biology Disorder? or Dynamics? 10 Å.

The Future of Structural Biology Disorder? or Dynamics? 10 Å.

Similar presentations

Ads by Google