1. Cell cycle, DNA, and replication

2. BIO… LIFE…. THINK ABOUT THIS: What do we do in our lifetime? What are the major stages of our life?

3. What are the Major stages of physical development in our lives?  What would it look like if we put this into a pie chart?

4. Imagine you are a bacteria: What are the major stages in your life? TRY ME:  What is A? B? C?  What is D/E/F/G collectively called?  What is D?  What is E?  What is F?  What is G?

5. The life of a cell  Cells have a life cycle just like we do- born, grow, reproduce, and die.  They only spend part of their life cycle reproducing (M)- mitosis

6. Cell Cycle 1. Interphase (G1, S, G2) – cell grows, prepares to divide (G phases) and DNA replicates (S phase) 2. Mitosis: nucleus divides; each nuclei winds up with the same # and kind of chromosomes as the parent (P-M-A-T) 3. Cytokinesis: division of cytoplasm

7. COMING UP IN THIS CLASS…  In the upcoming months, we will be analyzing what happens stages in great detail. Which stage do you think the following upcoming material will focus on? Topics to cover: -Cell cycle and events -DNA structure and replication? -DNA structure -Cell reproduction (asexual- bacteria)? -Cell reproduction (growth- multicellular)? -DNA and making proteins -Proteins and regulation of the cell cycle -What happens when things go wrong….

8. NUCLEIC ACIDS: The 4 th category of Biomolecules  What were the 4 macromolecules/biomolecules?  What are monomers? Polymers?  What is the name of the process that builds monomers to polymers called?  What is the reverse process called?

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10.  Nucleic Acids are the chemical link between generations dating back to the beginning of life on earth. 10

11.  A nucleic acid is a complex macromolecule that stores information in cells in the form of a code. 11

12. Terminology:  What is the difference and relationship between the following: -DNA-nucleus -Chromosomes- chromatid -Genes- chromatin -Centromere-histones -nucleotides 12

13. What are chromosomes??? 13 Nucleotides make up DNA DNA winds into Chromatin Chromatin condenses into Chromosomes are composed of chromatids held together at the centromere Chromosomes contain Genes Genes are sections of DNA DNA is made of nucleotides Chromosomes contain Instructions for making YOU!!!

14. Chromosome - DNA Chromosome- Chromatid Where is it located in the cell? NUCLEUS! How is it arranged?

15. Figure 9-4 Chromosome structure DNA double helix histone proteins DNA wound around histone proteins Loops attached to a protein scaffold; this stage of partial condensation typically occurs in a nondividing cell Coiled DNA/histone beads protein scaffold Folded chromosome, fully condensed in a dividing cell

17. NUCLEIC ACID  What is the monomer of nucleic acids called?  What components does it have?  What are the 2 major nucleic acids?  What do they do?  What ways are they similar in structure?  What 3 ways do they differ in structure? THEME….how is the structure related to their function? 17

18.  MONOMER: Nucleic acids are made of long chains of nucleotides.  Nucleotides are made of three components: 1. sugar 2. phosphate group 3. nitrogen base 18

19.  Examples of nucleic acids are : 1. DNA 2. RNA 19

20. DNA = deoxyribonucleic acid its components are: 1. deoxyribose (sugar) 2. phosphate group 3. nitrogen base 20 Purpose? To code for everything in your body...genetic blueprint!

21. The Components and Structure of DNA There are four kinds of bases in in DNA:  adenine  guanine  cytosine  thymine 21

22. Adenine (A) – Thymine (T) Guanine (G) – Cytosine (C) A and G are purines (AGgies eat Purina) C and T are pyrimidines 22

23.  The paired nitrogen bases formed two long strands of nucleotides that compliment each other. 23

24.  Nitrogen Bases are connected between sugars and phosphates  They declared, “This structure is a “double helix” The structure is antiparallel (upsidedown). This causes a problem in replication Because replication is a one way direction. 24

25. DNA Double Helix 25

26. WHO DISCOVERED THIS???  …..its complicated

27. IV. Watson and Crick (1953) /Wilkins and Franklin A. Watson and Crick 1. double helix model of DNA based on the work of other scientists B. Maurice Wilkins and Rosalind Franklin 1. x-ray diffraction photographs of DNA helped Watson and Crick develop their model 2. Watson, Crick and Wilkins received Nobel Prize in l962 (Franklin died in 1958 and could not be named for the award)

28. X-ray Diffraction Photo Rosalind Franklin of DNA) and Maurice Wilkens

29. DNA Double Helix Watson and Crick

30. V. Erwin Chargaff - 1949 CHARGAFF: Percent of nitrogen bases that pair with each other (complementary bases) is the same 1. percent adenine equals percent thymine percent guanine equals percent cytosine 2. implied base pairing rules

31.  DNA forms chromosomes, units of genetic information which pass from parent to offspring. DNA is wound into structures called chromosomes during cell division 31

32.  If you unraveled all your chromosomes from all of your cells and laid out the DNA end to end, the strands would stretch from the Earth to the Moon about 6,000 times. 32

33.  RNA has a different sugar than DNA  RNA = ribonucleic acid  It’s components are : 1. ribose (sugar) 2. phosphate group 3. nitrogen base 33

34.  It also has different bases than DNA adenine --- uracil cytosine --- guanine  RNA is also single stranded, not double stranded like DNA. 34

35. DNARNA - Double stranded- Single stranded - Sugar = deoxyribose - Sugar = ribose - Adenine pairs with Thymine - Adenine pairs with Uracil 35

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37. I. Why Must DNA Replicate?  Every time a cell divides, it must first make a copy of it’s chromosomes.  Therefore, each cell can have a complete set of chromosomes. 37

38.  Without replication, species could not survive and individuals could not successfully grow and reproduce. 38

39. II. How DNA Replicates  DNA is a molecule composed of TWO strands, each consisting of a sequence of nucleotides.  The order of the nitrogen bases on one strand mandates the sequence of bases on the complementary strand. 39

40. If you know the bases on one strand, you can predict which bases will occur on the complementary strand. A----- G ----- T----- C----- T----- During Replication each strand serves as a template to create a new strand. TCAGGGATCAGGGA 40

41. III. Steps In Replication 1) Helicase break down the hydrogen bonds between the two DNA strands, unzipping the molecule 41

42.  DNA Replication 1. Helicase break the hydrogen bonds between the bases so the chains of DNA can separate or unwind

43. 2) As the DNA unzips, SSB (single strand binding protein holds the strands down. 3) Primase(RNA) makes start platforms 43

44. 4) DNA polymerase adds nucleotides (from surroundings in the nucleus) bond to the single strands by base pairing (A-T, G- C) SEMI- CONSERVATIVE REPLICATION: ** The result of this process is the formation of TWO DNA molecules, each identical to the original molecule- with one old strand and one new strand.

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47. 2. Other enzymes add new nucleotides, which form new hydrogen bonds with their complimentary nucleotide. Red = original strand Blue = new strand  DNA Replication

48.  Results in 2 semi-conservative double stranded molecules

49. WHAT IS THE ROLE OF: Helicase- SSB- Replication fork- DNA Polymerase-

50. ANIMATIONS OF DNA REPLICATION http://www.bioteach.ubc.ca/TeachingResources/MolecularBiology/DNAReplication.s wf http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf http://highered.mcgraw- hill.com/sites/0072556781/student_view0/chapter11/animation_quiz_2.html http://www.sinauer.com/cooper/4e/animations0601.html AMEOBA SISTERS: https://www.youtube.com/watch?v=5qSrmeiWsuc&index=1&list=PLwL0Myd7Dk1F1p p-DaLx3ygO_7xA-yyd4

51. 1. What are the two types of nucleic acids? 2. What are the three components of a nucleotide? 3. What are the similarities between DNA and RNA? What are the differences? 4. Describe the process of DNA replication. 5. Why does a DNA molecule undergo replication? 51