1. BIOSAFETY LEVEL 2 AWARENESS TRAINING UW Stout Laboratory Safety Biological Safety

2. Introduction This training provides a basic understanding of biosafety hazards and practices required to enter UW-Stout’s designated biosafety level 2 (BSL-2) laboratory spaces It does not provide all required training (e.g., bloodborne pathogens, use of biosafety cabinets, standard microbiological practices) for work with agents in BSL-2 containment It is a supplement to, not a replacement for, the Laboratory Safety Training modules on D2L

3. What Does “Biosafety Level 2” Mean? The biosafety level (BSL) defines the laboratory practices and techniques, safety equipment and laboratory facilities recommended for work with biohazardous materials. UW-Stout has facilities for working with materials that require BSL-1 or BSL-2 containment:  BSL-1: For work with defined and characterized strains of viable microorganisms not known to consistently cause disease in healthy adult humans  BSL-2: For work with moderate-risk agents that are associated with human disease of varying severity

4. What are “Biohazardous Materials”? Biohazardous materials are infectious agents or hazardous biological materials that present a potential risk to the health of humans, animals or the environment. They include:  Recombinant DNA  Organisms that can affect health (e.g., parasites, bacteria, fungi, viruses)  Biologically active agents (e.g., toxins, allergens, venoms)

5. How Do I Know Which are BSL-2 Labs? All doors to laboratory or classroom spaces that contain materials being worked with using BSL-2 criteria will have the sign shown on the right The sign advises of entry and exit requirements that must be followed by everyone who enters the space, not just people working with the materials Current BSL-2 Labs in Jarvis are 247, 249, 257 and 267

6. Can I Do Other Work in a BSL-2 Lab? Multi-use labs may contain areas that are not to be used for BSL-2 work; these areas are designated with blue washable tape placed along the perimeter of the work surfaces These blue-labeled areas must not be used for BSL-2 work, and will not require decontamination specific to BSL-2 areas Equipment from blue-labeled areas can be moved to and from non-BSL-2 areas without decontamination Everyone entering the lab should familiarize themselves with the location of the non-BSL-2 spaces

7. What are the Hazards? Biohazardous materials that require BSL-2 containment have a moderate risk of causing human disease Exposure could occur from:  Accidental needle stick or other skin puncture  Splash into eyes, nose, mouth  Accidental ingestion If handled carefully (using standard microbiological practices) these materials can be used safely on open benches  Procedures that may create infectious aerosols or splashes should be performed in a biological safety cabinet The risk of being exposed by being in the same room is extremely low.

8. What are the Rules? Lab coats are mandatory No open-toed shoes Safety glasses must be worn by everyone if someone in the lab is working with a liquid chemical or biohazardous material Chemical splash goggles (indirectly vented or un-vented; NOT vented impact goggles) must be worn by people working with a biohazardous material if there is a possibility of creating a splash Lab doors must not be propped open Eating, drinking, smoking, handling contact lenses, applying cosmetics and storing food for human consumption are not allowed at any time Hands must be washed with soap and water before leaving the room

9. What Do I Do With My Lab Coat? Lab coats should always be left in the lab Lab coats will be laundered for you at the end of each semester, at a minimum Do not take personal lab coats home until after they have been laundered (talk to your instructor or lab manager for confirmation) If your lab coat is visibly contaminated with biological materials, it should be autoclaved and laundered immediately

10. This concludes the BSL-2 Awareness Training Module Please complete the BSL-2 Entry Quiz Requirement, Quiz 12 – BSL-2 Entry Conclusion