Presentation is loading. Please wait.

Presentation is loading. Please wait.

Humanization Phagemid Construction Large-scale. Humanization Light chainHeavy chain A4.6.1 (murine anti-VEGF antibody) Y0317 (anti-VEGF antibody Fab fragment)

Published byClaude Russell Modified over 9 years ago

Similar presentations

Presentation on theme: "Humanization Phagemid Construction Large-scale. Humanization Light chainHeavy chain A4.6.1 (murine anti-VEGF antibody) Y0317 (anti-VEGF antibody Fab fragment)"— Presentation transcript:

1 Humanization Phagemid Construction Large-scale

2 Humanization Light chainHeavy chain A4.6.1 (murine anti-VEGF antibody) Y0317 (anti-VEGF antibody Fab fragment) humanized

3 Phagemid construction phoA  alkaline phosphatase promoter stII  periplasm secretion signal geneIII  coat proteins of M13 bacteriophage Light chain Heavy chain

4 Periplasm 12~15 nm Gram negative bacteria cell wall Contains only 4% of the total cell protein Easy purification by osmotic shock Oxidizing environment facilitates the formation of disulfide bond periplasm

5 E. coli strain engineering Overexpress chaperone  Protein folding –GroEL+GroES –KJE complex (DnaK+DnaJ & GrpE) Overexpress disulfide bond enzyme  Forming disulfide bond –DsbA Lower protease  Inhibit protein degradation

6 Alkaline phosphatase promoter Growth time Cell number Fast growth High phosphate Low phosphate Induction High phosphate medium Low expression (cell growth) NO Need Inducer!! Low phosphate medium High expression (induction)

Download ppt "Humanization Phagemid Construction Large-scale. Humanization Light chainHeavy chain A4.6.1 (murine anti-VEGF antibody) Y0317 (anti-VEGF antibody Fab fragment)"

Similar presentations

Production of Human Growth Hormone in Genetically Modified Bacteria

Production of Human Growth Hormone in Genetically Modified Bacteria
1. Complement System 2. Antigen Specific Receptors K.J. Goodrum Department of Biomedical Sciences 2005.

1. Complement System 2. Antigen Specific Receptors K.J. Goodrum Department of Biomedical Sciences 2005.
Cell-free Bacterial Yeast Insect Mammalian Protein Expression Systems.

Cell-free Bacterial Yeast Insect Mammalian Protein Expression Systems.
Manifestation of Novel Social Challenges of the European Union in the Teaching Material of Medical Biotechnology Master’s Programmes at the University.

Manifestation of Novel Social Challenges of the European Union in the Teaching Material of Medical Biotechnology Master’s Programmes at the University.
A genetic switch with memory: the lysis/lysogeny switch in phage 

A genetic switch with memory: the lysis/lysogeny switch in phage 
Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA Chao WANG Sept. 13, 2006.

Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA Chao WANG Sept. 13, 2006.
Process design for the production of Fab fragment for therapy Group 6 Shiang-Yuan Hsieh, Yun-Hsuan Liu, Chao-Ming Yen.

Process design for the production of Fab fragment for therapy Group 6 Shiang-Yuan Hsieh, Yun-Hsuan Liu, Chao-Ming Yen.
Research Techniques Made Simple: Antibody Phage Display Christoph M. Hammers and John R. Stanley Dept. of Dermatology, University of Pennsylvania, Philadelphia,

Research Techniques Made Simple: Antibody Phage Display Christoph M. Hammers and John R. Stanley Dept. of Dermatology, University of Pennsylvania, Philadelphia,
Humoral Immunity.

Humoral Immunity.
Three major factors influence protein expression Host Growth Conditions Vector Thus, you should consider the solutions for YOUR expression problems at.

Three major factors influence protein expression Host Growth Conditions Vector Thus, you should consider the solutions for YOUR expression problems at.
“Folding quality control in the export of proteins by the bacterial twin-arginine translocation pathway” DeLisa MP, Tullman D, Georgiou G. Proc Natl Acad.

“Folding quality control in the export of proteins by the bacterial twin-arginine translocation pathway” DeLisa MP, Tullman D, Georgiou G. Proc Natl Acad.
ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 The Cell as a Production Factory, Expression Systems.

ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 The Cell as a Production Factory, Expression Systems.
1 Genetic regulation Genotype is not phenotype: bacteria possess many genes that they are not using at any particular time. Transcription and translation.

1 Genetic regulation Genotype is not phenotype: bacteria possess many genes that they are not using at any particular time. Transcription and translation.
Gram-Positive DegP Evaluation

Gram-Positive DegP Evaluation
Bacterial Physiology (Micr430)

Bacterial Physiology (Micr430)
 What actually happens inside the cell in response to genetic engineering, not just how we manipulate and alter cell  Can use to predict responses of.

 What actually happens inside the cell in response to genetic engineering, not just how we manipulate and alter cell  Can use to predict responses of.
Foreign Gene Expression and Protein Production in Prokaryotes and Eukaryotes Prokaryotic Expression Systems Fusion Proteins Biofilms Secretion Eukaryotic.

Foreign Gene Expression and Protein Production in Prokaryotes and Eukaryotes Prokaryotic Expression Systems Fusion Proteins Biofilms Secretion Eukaryotic.
Introduction recombinant expression of protein disulfide isomerase (PDI) using the model plant Arabidopsis thaliana Eun Ju Cho ABE workshop 2007.

Introduction recombinant expression of protein disulfide isomerase (PDI) using the model plant Arabidopsis thaliana Eun Ju Cho ABE workshop 2007.
Genetic transformation of E. coli bacteria

Genetic transformation of E. coli bacteria
Recombinant protein expression. Other alternatives

Recombinant protein expression. Other alternatives

Similar presentations

Ads by Google