1. ProStar 335 Detector Wednesday, 26 April 2017
Petro van Poppel – Field Support Specialist
Wednesday, 26 April 2017

2. Overview Production Tests with 0x0 flow cell
Introduction to PS 335 Detector
Dual Array option
Poly Verify Software
Flow cell
Communications
Poly Verify
Production Tests with 0x0 flow cell
Wavescan
(Settings Tab)
(Input/Output Tab)
(EEPROM)
(COMMS Tab)
(Calibration Tab)
PS 335 Shipping Test
Problems

3. ProStar 335 Mechanical Optical layout

4. ProStar 335 Ray Trace Layout

5. 335 Optics

6. 335 Ray Trace

7. 335 Single and Dual Array
Single Array 1024 photodiode sample array 2 photodiode references (250nm and 500 nm) No extended range spectra Uses only 9x0 and 4x0 flow cells Microbore to Analytical applications Dual Array 1024 photodiode sample and reference array Extended range spectra (up to AU) Used for analytical to prep apps

8. Flow Cell Ray Beam General design of the 9x0 flow cell

9. Flow Cell Specs Flow Cell Prep (9 x 1) Super Prep (4 x 0.15)
Analytical
(9 x 0)
Micro
(4 x 0 )
Pressure rating
1000 psi
Flow Cell Volume
30 µl
145 µl
15 µl
1.5 µl
Material (non optical)
Ti & PEEK
Noise Performance
200 ml/min
10-500ml
1x10-4AU
800 ml/min L
40 ml/min
0.1 µl-100 ml
5x10-3AU
1 ml/min
0-20 ml
7.5x10-3AU

10. Communications TCP/IP communications for Detector: Static or Dynamic
Networked or standalone
Firewall
Access on Network, OS or PC

11. PS 335 PolyVerify Software

12. PS 335 PolyDiagnostics Ctrl-Shift-F10 to enable Diagnostics side
Ctrl-Shift-F9 to hide Diagnostics side

13. 335 PolyVerify Test Certificate
Test Certificate provided by the factory for each 335 detector

14. PS 335 PolyVerify Tests

15. PS 335 Noise Test

16. PS 335 Wavelength Accuracy test
Run with a 4 nm slit

17. PS 335 SBW Accuracy Test
Wavelength Range of 190 to 950 nm

18. PS 335 Detector Linearity Test
pump head pressure of at least 100 atm using a back pressure restrictor of 40psi .

19. PS 335 Wavescan

20. PS 335 Wavescan Results
This wavescan seems to have a rather large difference between the sample and reference paths (long and short paths). Possible reasons for this are bad calibration, dirty flow cell, air bubbles in flow cell, foggy optics.

21. PS 335 Contributions from each Lamp

22. PS 335 PolyDiagnostics Mode

23. PS 335 Input/Output Mode

24. PS 335 Main PWB Power Supply LED’sC
A: + 24 V power Supply
B: + 9V and – 9V Power Supply
C: + 5 V Power Supply
Front of the Unit

25. PS 335 Identifying the Array Type
Knowing the Array type is necessary when running the Input/Output test diagnostics

26. PS 335 Dual Array P8 disconnect
P8 connection

27. PS 335 PolyDiagnostics EEPROM

28. PS 335 PolyDiagnostics COMMS

29. PS 335 PolyDiagnostics Calibration Tab

30. Caffeine Shipping Test
PS 335 Caffeine Shipping Test:
Test made up of 1 components:
Loop injection using 25 µg/ml caffeine
Test is conducted using Galaxie Software

31. PS 325/335 Caffeine Loop Injection Test
Test Calculates:
Peak Height
Peak Area
Noise
S/N

32. Common Problems PS 335 Error Messages Table can be found: High Noise
Lamp on, not ready
SIG_CC_SLIT_PEAK_NOT_FOUND
SIG_CC_SIGNAL_OVERRANGE_REPREFORM_ LAMP_CAL
COM_CC_CIRCULAR_BUFFER_OVERRUN _OCCURED
9555 LMP_CC_D2_FILAMENT_FAILURE
Foggy Optics
PS 335 Error Messages Table can be found:
PS 335 Service Manual
Dealernet

33. Foggy Optics
What is Foggy Optics?

34. What is Foggy Optics? Three types of Foggy optics
White powdery coating
Rust/brown spot burn
Speckled mirror (delamination)

35. What is Foggy Optics? Diagnosing Foggy Optics

36. Diagnosing Foggy Optics
Run a “wavescan” test, When bad Open the optics and inspect.

37. Wavescan test results Intensity for the 254 nm line
Currently is 10 – 50 K counts

38. False positive
New D2 vs Aged D2 Intensities

39. What is Foggy Optics? Diagnosing Foggy Optics Treating Foggy optics

40. 0603 Project (Foggy Optics)
Proposed Fixes and their status:
White powder fogging: due to leaky flow cells and customer sample depositing on the mirrors
New Flow cells & fittings available for all cells except 4 x 0.15
Brown powder fogging: due to UV attack on some of the foam parts close to the UV source.
Eliminating as much of the foam as possible and shielding the rest.
Mirror delamination: due to UV attack on the thin Al coating of the mirrors
Eliminated by putting a NiCr coating on the mirrors to strengthen the Al coating.

41. Opening and cleaning the optics

42. Cleaning 325 optics
Clean area in circle
Do not adjust!!

43. Wavelength calibration data must be entered into the 335 EEPROM
Replacing Optics
Wavelength calibration data must be entered into the 335 EEPROM

44. Replacing Optics
This label is attached to the top of each optics module, the shipping box and the test document

45. Summary Single and Dual Array Optical path is open
Flow cell is dual path
Diagnostics Tests, like wavescan
Shipping Test
Main Problems (Foggy Optics)