1. CHARACTERIZATION OF NATURAL AND ENGINEERED NANOPARTICLES: SHAPE, SIZE AND CHEMICAL COMPOSITION Lucia Manangon http://www.theismaili.org/cms/317/Building-the-future-one-nanoparticle-at-a-time

2. NANOPARTICLES Ferrihydrite Natural nanoparticle CNT 0.25 µm 100 nm TEM image of 20 nm Au NP Engineered nanoparticle Poda et al., J. Chromatogr. A. 2010, 1218 (27), 4219-4225.

3. NANOPARTICLES IN COMERCIAL PRODUCTS FATE ?? BEHAVIOR ?? BIOAVAILABILITY ?? TOXICITY ??

4. Characterization and Analytical Techniques Size Shape Composition Field Flow fractionation (FFF) Multi Angle Light Scattering (MALS) Dynamic Light Scattering (DLS) Inductively coupled Plasma (FFF- ICP-MS)

5. FFF FRACTIONATION

6. MULTI ANGLE LIGHT SCATTERING (MALS) – DYNAMIC LIGHT SCATTERING (DLS) incident beam scattered light SIZE AND SHAPE

7. ICP-CC-MS Li C Na Mg Al Si P S K Ca Ti Mn Fe Co Ni Cu Zn As Se Sr Mo Ag Sb Cs Hg Pb U 27 elements are analyzed: CHEMICAL COMPOSITION

8. Online system ICP - MS FFF UV MALS - DLS FLUORESCENCE

9. SAMPLES  Au, Ag  PS microspheres  CNT Natural nanoparticle Engineered nanoparticle GSL  Pore water  Deep brine layer  Deep brine sediment FRACTIONATIONSIZE AND SHAPE CHEMICAL COMPOSITION UNDERSTANDING OF FATE, BEHAVIOR, BIOAVAILABILITY AND TOXICITY